Use of MALDI-TOF mass spectrometry for virus identification: a review
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F22%3APU144917" target="_blank" >RIV/00216305:26620/22:PU144917 - isvavai.cz</a>
Alternative codes found
RIV/62156489:43210/22:43921604
Result on the web
<a href="https://doi.org/10.1039/D2AN00431C" target="_blank" >https://doi.org/10.1039/D2AN00431C</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/d2an00431c" target="_blank" >10.1039/d2an00431c</a>
Alternative languages
Result language
angličtina
Original language name
Use of MALDI-TOF mass spectrometry for virus identification: a review
Original language description
The coronavirus pandemic caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) directly or indirectly affects every individual worldwide. The fight against SARS-CoV-2 is based on the rapid and accurate diagnosis and subsequent isolation of infected individuals. Therefore, the demands for the scientific development of diagnostic methods for the confirmation of SARS-CoV-2 are enormous. Currently, reverse-transcription quantitative polymerase chain reaction (RT-qPCR) is the main method used for detecting viruses, including SARS-CoV-2, and is considered the gold standard for coronavirus disease 2019 (COVID-19) identification. However, various alternatives have been investigated due to the time and cost demands of this method or to shortages of reagents. In this review, we focus on matrix-assisted laser desorption and ionisation with time-of-flight analyser mass spectrometry (MALDI-TOF MS) techniques as potential tools for the diagnosis of viruses with an emphasis on SARS-CoV-2. MALDI-TOF is commonly used in clinical laboratories for bacterial characterization and identification, but in the field of clinical virology, MALDI-TOF remains only a promising technology for routine diagnosis. This review provides an overview of the development of clinical virology from the point of view of using MALDI-TOF for virus identification and as a possible diagnostic tool for SARS-CoV-2 detection. In addition, this review summarizes the current state of standard methods for virus diagnostics including the preparation of clinical samples.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ANALYST
ISSN
0003-2654
e-ISSN
1364-5528
Volume of the periodical
147
Issue of the periodical within the volume
14
Country of publishing house
GB - UNITED KINGDOM
Number of pages
24
Pages from-to
3131-3154
UT code for WoS article
000812310800001
EID of the result in the Scopus database
2-s2.0-85132519114