Engineering Conductive Hydrogels with Tissue-like Properties: A 3D Bioprinting and Enzymatic Polymerization Approach

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F24%3APU152214" target="_blank" >RIV/00216305:26620/24:PU152214 - isvavai.cz</a>

Result on the web

<a href="https://onlinelibrary.wiley.com/doi/10.1002/smsc.202400290" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1002/smsc.202400290</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/smsc.202400290" target="_blank" >10.1002/smsc.202400290</a>

Result language

angličtina

Original language name

Engineering Conductive Hydrogels with Tissue-like Properties: A 3D Bioprinting and Enzymatic Polymerization Approach

Original language description

Hydrogels are promising materials for medical devices interfacing with neural tissues due to their similar mechanical properties. Traditional hydrogel-based bio-interfaces lack sufficient electrical conductivity, relying on low ionic conductivity, which limits signal transduction distance. Conducting polymer hydrogels offer enhanced ionic and electronic conductivities and biocompatibility but often face challenges in processability and require aggressive polymerization methods. Herein, we demonstrate in situ enzymatic polymerization of pi-conjugated monomers in a hyaluronan (HA)-based hydrogel bioink to create cell-compatible, electrically conductive hydrogel structures. These structures were fabricated using 3D bioprinting of HA-based bioinks loaded with conjugated monomers, followed by enzymatic polymerization via horseradish peroxidase. This process increased the hydrogels' stiffness from about 0.6 to 1.5 kPa and modified their electroactivity. The components and polymerization process were well-tolerated by human primary dermal fibroblasts and PC12 cells. This work presents a novel method to fabricate cytocompatible and conductive hydrogels suitable for bioprinting. These hybrid materials combine tissue-like mechanical properties with mixed ionic and electronic conductivity, providing new ways to use electricity to influence cell behavior in a native-like microenvironment. This study introduces a novel method to enhance hydrogel conductivity and biocompatibility for biomedical applications. By using in situ enzymatic polymerization of pi-conjugated monomers within a hyaluronan-based hydrogel bioink, followed by 3D bioprinting, the resulting hydrogels exhibit improved stiffness, electroactivity, and cytocompatibility. These conductive hydrogels provide a versatile platform for advanced 3D cell culture and neural engineering.image (c) 2024 WILEY-VCH GmbH

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

21000 - Nano-technology

Project

<a href="/en/project/GA24-10775S" target="_blank" >GA24-10775S: Biopotential Recordings with Flexible ITO Field Effect Transistors (BioFlexFET)</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

SMALL SCIENCE

ISSN

2688-4046

e-ISSN

—

Volume of the periodical

4

Issue of the periodical within the volume

11

Country of publishing house

US - UNITED STATES

Number of pages

12

Pages from-to

„“-„“

UT code for WoS article

001303017000001

EID of the result in the Scopus database

2-s2.0-85202748555