Open-channel microfluidic device for TiO2NTs@Fe3O4NPs-assisted viral RNA extraction and amplification-free RNA fluorescence status evaluation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F24%3APU152640" target="_blank" >RIV/00216305:26620/24:PU152640 - isvavai.cz</a>

Alternative codes found

RIV/00179906:_____/24:10485741 RIV/00216224:14330/24:00139280 RIV/00216208:11150/24:10485741 RIV/00216208:11160/24:10485741 RIV/00216275:25310/24:39921560

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0026265X24016667?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0026265X24016667?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.microc.2024.111554" target="_blank" >10.1016/j.microc.2024.111554</a>

Result language

angličtina

Original language name

Open-channel microfluidic device for TiO2NTs@Fe3O4NPs-assisted viral RNA extraction and amplification-free RNA fluorescence status evaluation

Original language description

Most routine viral RNA detection assays available today rely on RNA amplification steps. The sensitivity of such methods comes at the expense of time, cost, and equipment availability. Given the constraints during the COVID-19 pandemic, such as the limited capacity of RNA amplification instruments and the global shortage of isolation kits, there has been a demand for amplification-free assays which should meet POCT criteria: affordable, sensitive, specific, user-friendly, rapid, and equipment-free. This study aims to design and validate a microanalytical system for direct viral RNA detection which meets these criteria. The presented RNA detection assay consists of four consecutive steps: immunocapturing viral particles, viral particles lysis, TiO2NTs@Fe(3)O(4)NPs-assisted RNA extraction, and finally viral RNA detection. The last three steps occur within a microfluidic device with open channels (OC-MFD). The OC-MFD is manufactured from polycarbonate film by unique mechanical replication techniques in a roll-to-roll setup. This method has been experimentally validated to offer the advantages of low-cost and extremely rapid production (1 mil. pieces of OC-MFDs per hour). Two permanent magnets situated under the application/extraction zones enabled to fix magnetically active particles/nanotubes. At first, viral particles are quantitatively captured from the entire sample volume using immunomagnetic particles. The lysed viral particles are transferred to the application zone of OC-MFD, followed by RNA extraction using magnetic TiO2 nanotubes decorated with Fe3O4 nanoparticles (TiO2NTs@Fe(3)O(4)NPs). The effectivity of the nanomaterial to attract viral RNA molecules was evaluated against a standard RT-qPCR-based laboratory test. Equivalence tests were performed with 143 positive and negative SARS-CoV-2 samples. Spearman rank correlation coefficients demonstrated high agreement for both measured biomarker genes (the E gene equaled 0.76, the RdRp gene equaled 0.73, the p-va

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10400 - Chemical sciences

Project

<a href="/en/project/EF17_048%2F0007421" target="_blank" >EF17_048/0007421: Strengthening interdisciplinary cooperation in research of nanomaterials and their effects on living organisms (NANOBIO)</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

MICROCHEMICAL JOURNAL

ISSN

0026-265X

e-ISSN

1095-9149

Volume of the periodical

206

Issue of the periodical within the volume

111554

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

13

Pages from-to

„“-„“

UT code for WoS article

001317526400001

EID of the result in the Scopus database

2-s2.0-85203496386