On-demand in situ hydrogen peroxide generation using red light-A new tool for in vitro redox biology
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F26%3A0198688" target="_blank" >RIV/00216305:26620/26:0198688 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0891584925005192?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0891584925005192?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.freeradbiomed.2025.05.244" target="_blank" >10.1016/j.freeradbiomed.2025.05.244</a>
Alternative languages
Result language
angličtina
Original language name
On-demand in situ hydrogen peroxide generation using red light-A new tool for in vitro redox biology
Original language description
Hydrogen peroxide (H2O2) is the most stable form of reactive oxygen species involved in essential cellular processes such as redox signaling, metabolic regulation, and stress response. Conventional delivery methods, such as direct addition or chemical precursors, provide limited spatial and temporal control, making it challenging to study its precise physiological or pathological effects. To address this, there is a growing demand for spatiotemporal H2O2 delivery systems in redox biology. Here, we report on our efforts to create devices that photoelectrochemically reduce dissolved oxygen to hydrogen peroxide, while consuming various organic molecules as electron donors. In this study, we introduce a platform for in situ generation of H2O2 using red light-activated devices available in two configurations: a planar version, that can be integrated onto glass slides or cell culture plates, and a microstructured injectable suspension, which can be introduced into an experimental system as needed. The devices are composed of a gold layer and organic semiconductor materials, with thicknesses of less than 100 nm. Upon illumination, the organic semiconductor side facilitates H2O2 formation through an oxygen reduction reaction, while the gold layer simultaneously enables the oxidation of an electron donor present in the surrounding medium. The devices can provide reliable H2O2 production in complex cell culture media and can be easily used in multiwell plates. This system provides a controllable and non-invasive method for localized H2O2 delivery driven by light illumination.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
10600 - Biological sciences
Result continuities
Project
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Continuities
R - Projekt Ramcoveho programu EK
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů