Surface plasmon resonance imaging - first experience

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00843989%3A_____%2F14%3AE0104495" target="_blank" >RIV/00843989:_____/14:E0104495 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Surface plasmon resonance imaging - first experience
Original language description
Aim of the study were to optimize the preparation of biochips, set up and launch a new SPRi-Lab+ ™ device and SPRi-Arrayer spotting equipment (by HORIBA company). For the given task, pilot method for the ovalbuminu (OVA) determination was chosen. Surface plasmon resonance imaging (SPRi) can be used for the determination of biomolecules on the surface of the chip (prism with nanolayer deposited gold) in real time, without labeling any of the reactants. Analysers applying this technique use CCD camera for sensing of the biochip surface (microscopic image of surface). The preparation of biochips by using the SPRi-Arrayer equipment and determination of ovalbumin were optimized. Analytical characteristics of this method were statistically established. The limit of detection (0.0041 mg.l-1) based on measurements of the blank, sensitivity of the method and detection limit of the calibration dependence were determined. Method for the determination of ovalbumin gives reliable results in a concentration ranging from 0.01 to 10.00 mg.l-1.
Czech name
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Czech description
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Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
CECE 2014. 11th International Interdisciplinary Meeting on Bioanalysis. October 20-22, 2014
ISBN
978-80-904959-2-0
ISSN
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e-ISSN
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Number of pages
4
Pages from-to
390-393
Publisher name
Institut of Analytical Chemistry ASCR
Place of publication
Brno
Event location
Brno, Czech Republic
Event date
Oct 20, 2014
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
000354547400126

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