OralDisk: A chair-side compatible molecular platform using whole saliva for monitoring oral health at the dental practice

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00843989%3A_____%2F21%3AE0109243" target="_blank" >RIV/00843989:_____/21:E0109243 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14160/21:00124580 RIV/61988987:17110/21:A2302FAH

Result on the web

<a href="https://www.mdpi.com/2079-6374/11/11/423" target="_blank" >https://www.mdpi.com/2079-6374/11/11/423</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/bios11110423" target="_blank" >10.3390/bios11110423</a>

Result language

angličtina

Original language name

OralDisk: A chair-side compatible molecular platform using whole saliva for monitoring oral health at the dental practice

Original language description

Periodontitis and dental caries are two major bacterially induced, non-communicable diseases that cause the deterioration of oral health, with implications in patients' general health. Early, precise diagnosis and personalized monitoring are essential for the efficient prevention and management of these diseases. Here, we present a disk-shaped microfluidic platform (OralDisk) compatible with chair-side use that enables analysis of non-invasively collected whole saliva samples and molecular-based detection of ten bacteria: seven periodontitis-associated (Aggregatibacter actinomycetemcomitans, Campylobacter rectus, Fusobacterium nucleatum, Prevotella intermedia, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola) and three caries-associated (oral Lactobacilli, Streptococcus mutans, Streptococcus sobrinus). Each OralDisk test required 400 µL of homogenized whole saliva. The automated workflow included bacterial DNA extraction, purification and hydrolysis probe real-time PCR detection of the target pathogens. All reagents were pre-stored within the disk and sample-to-answer processing took < 3 h using a compact, customized processing device. A technical feasibility study (25 OralDisks) was conducted using samples from healthy, periodontitis and caries patients. The comparison of the OralDisk with a lab-based reference method revealed a ~90% agreement amongst targets detected as positive and negative. This shows the OralDisk's potential and suitability for inclusion in larger prospective implementation studies in dental care settings.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30208 - Dentistry, oral surgery and medicine

Project

—

Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biosensors

ISSN

2079-6374

e-ISSN

2079-6374

Volume of the periodical

11

Issue of the periodical within the volume

article 423

Country of publishing house

CH - SWITZERLAND

Number of pages

21

Pages from-to

1-21

UT code for WoS article

000784464100001

EID of the result in the Scopus database

2-s2.0-85118535345