Length-dependent translation efficiency of ER-destined proteins
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00843989%3A_____%2F23%3AE0110364" target="_blank" >RIV/00843989:_____/23:E0110364 - isvavai.cz</a>
Alternative codes found
RIV/61988987:17110/23:A2402N76
Result on the web
<a href="https://www.mdpi.com/1467-3045/45/8/425" target="_blank" >https://www.mdpi.com/1467-3045/45/8/425</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/cimb45080425" target="_blank" >10.3390/cimb45080425</a>
Alternative languages
Result language
angličtina
Original language name
Length-dependent translation efficiency of ER-destined proteins
Original language description
Gene expression is a fundamental process that enables cells to produce specific proteins in a timely and spatially dependent manner. In eukaryotic cells, the complex organization of the cell body requires precise control of protein synthesis and localization. Certain mRNAs encode proteins with an N-terminal signal sequences that direct the translation apparatus toward a specific organelle. Here, we focus on the mechanisms governing the translation of mRNAs, which encode proteins with an endoplasmic reticulum (ER) signal in human cells. The binding of a signal-recognition particle (SRP) to the translation machinery halts protein synthesis until the mRNA-ribosome complex reaches the ER membrane. The commonly accepted model suggests that mRNA that encodes a protein that contains an ER signal peptide continuously repeats the cycle of SRP binding followed by association and dissociation with the ER. In contrast to the current view, we show that the long mRNAs remain on the ER while being translated. On the other hand, due to low ribosome occupancy, the short mRNAs continue the cycle, always facing a translation pause. Ultimately, this leads to a significant drop in the translation efficiency of small, ER-targeted proteins. The proposed mechanism advances our understanding of selective protein synthesis in eukaryotic cells and provides new avenues to enhance protein production in biotechnological settings.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Current issues in molecular biology
ISSN
1467-3037
e-ISSN
1467-3045
Volume of the periodical
45
Issue of the periodical within the volume
8
Country of publishing house
CH - SWITZERLAND
Number of pages
11
Pages from-to
6717-6727
UT code for WoS article
001119117800001
EID of the result in the Scopus database
2-s2.0-85169039218