Retinal to Retinal Energy Transfer in a Bistable Microbial Rhodopsin Dimer
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F10974938%3A_____%2F25%3A25_88_13" target="_blank" >RIV/10974938:_____/25:25_88_13 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1021/jacs.5c01276" target="_blank" >https://doi.org/10.1021/jacs.5c01276</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/jacs.5c01276" target="_blank" >10.1021/jacs.5c01276</a>
Alternative languages
Result language
angličtina
Original language name
Retinal to Retinal Energy Transfer in a Bistable Microbial Rhodopsin Dimer
Original language description
Neorhodopsin (NeoR) is a newly discovered fungal bistable rhodopsin that reversibly photoswitches between UV- and near-IR absorbing states denoted NeoR367 and NeoR690, respectively. NeoR367 represents a deprotonated retinal Schiff base (RSB), while NeoR690 represents a protonated RSB. Cryo-EM studies indicate that NeoR forms homodimers with 29 Å center-to-center distance between the retinal chromophores. UV excitation of NeoR367 takes place to an optically allowed S3 state of 1Bu+ symmetry, which rapidly converts to a low-lying optically forbidden S1 state of 2Ag– symmetry in 39 fs, followed by a multiexponential decay to the ground state on the 1–100 ps time scale. A theoretically predicted nπ* (S2) state does not get populated in any appreciable transient concentration during the excited-state relaxation cascade. We observe an intradimer retinal to retinal excitation energy transfer (EET) process from the NeoR367 S1 state to NeoR690, in competition with photoproduct formation. To quantitatively assess the EET mechanism and rate, we experimentally addressed and modeled the EET process under varying NeoR367-NeoR690 photoequilibrium conditions and determined the EET rate at (200 ps)−1. The NeoR367 S1 state shows a weak stimulated emission band in the near-IR around 700 nm, which may result from mixing with an intramolecular charge-transfer (ICT) state, enhancing the transition dipole moment of the S1–S0 transition and possibly facilitating the EET process. We suggest that EET may bear general relevance to the function of bistable multiwavelength rhodopsin oligomers.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/GM21-09692M" target="_blank" >GM21-09692M: Clarifying quantum limits in biomolecules by utilizing entangled photons generated from protein bound cofactor modeled on orange carotenoid protein</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of the American Chemical Society
ISSN
0002-7863
e-ISSN
1520-5126
Volume of the periodical
147
Issue of the periodical within the volume
17
Country of publishing house
US - UNITED STATES
Number of pages
13
Pages from-to
14468−14480
UT code for WoS article
001469200600001
EID of the result in the Scopus database
2-s2.0-105002794901