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Identification of freshwater zooplankton species using protein profiling and principal component analysis

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25173154%3A_____%2F18%3AN0000007" target="_blank" >RIV/25173154:_____/18:N0000007 - isvavai.cz</a>

  • Alternative codes found

    RIV/60461373:22320/18:43915515 RIV/60461373:22330/18:43915515 RIV/60461373:22340/18:43915515

  • Result on the web

    <a href="https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.1002/lom3.10238" target="_blank" >https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.1002/lom3.10238</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1002/lom3.10238" target="_blank" >10.1002/lom3.10238</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Identification of freshwater zooplankton species using protein profiling and principal component analysis

  • Original language description

    Crustaceans are vital components of the freshwater zooplankton that link various trophic levels. Because crustaceans, particularly cladocerans of the Daphnia genus, are sensitive to environmental changes, they can serve as indicators of environmental fluctuations. Therefore, it is highly desirable to have a fast and reliable method for the identification of individual Daphnia species. In this study, we demonstrated the ability of protein profiling to distinguish between freshwater zooplankton species. Individual specimens were morphologically identified before being analyzed by proteomic fingerprinting using matrix‐assisted laser desorption/ionization time of flight mass spectrometry (MALDI‐TOF MS). The obtained mass spectra were used to create a reference library. Subsequently, the individual species were successfully identified using Biotyper software. The variability between the spectra of individual Daphnia species was verified by principal component analysis (PCA). Our results suggest that the combination of proteome fingerprinting using MALDI‐TOF MS and PCA has considerable potential as a rapid tool for the unambiguous identification of individual species of freshwater zooplankton without the need for expert morphological analysis.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10503 - Water resources

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2018

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Limnology and Oceanography: Methods

  • ISSN

    1541-5856

  • e-ISSN

  • Volume of the periodical

    16

  • Issue of the periodical within the volume

    3

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    6

  • Pages from-to

    199-204

  • UT code for WoS article

    000428449500004

  • EID of the result in the Scopus database

    2-s2.0-85041180794&