Micropropagation of blackberry genotypes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F16%3AN0000007" target="_blank" >RIV/25271121:_____/16:N0000007 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.17660/ActaHortic.2016.1133.75" target="_blank" >http://dx.doi.org/10.17660/ActaHortic.2016.1133.75</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17660/ActaHortic.2016.1133.75" target="_blank" >10.17660/ActaHortic.2016.1133.75</a>

Result language

angličtina

Original language name

Micropropagation of blackberry genotypes

Original language description

The purpose of this study was to develop an efficient in vitro multiplication system for blackberry (Rubus fruticosus L.). A successful in vitro culture technique would provide an alternative method, which can potentially multiply selected blackberry genotypes more rapidly than traditional nursery systems. Two genotypes, 'Cacanska bestrna' and blackberry selection No. 7, were sterilised using 0.15% mercuric chloride as sterilization solution. The use of mercuric chloride had a direct beneficial effect to overcome the contamination from all starting plant material. Four types of modified MS medium containing 1, 2 or 4 mg L-1 6-benzylaminopurine (BAP) or combination of 1 mg L-1 BAP, 0.1 mg L-1 indole-3-butyric acid (IBA) and 0.1 mg L-1 gibberellic acid GA (3) were tested in multiplication stage. Generally, the highest proliferation rate was obtained for cultivar Tatanska bestrna' that produced 6.3 shoots (longer than 10 mm) on a medium containing 4 mg L-1 BAP. All in vitro shoots started spontaneous rooting on multiplication media after depletion of cytokinines after 1 month ofcultivation. High survival (more than 95%) was obtained after acclimatization. The plants established after a period of in vitro culture showed no visible morphological differences compared with conventionally propagated plants. In conclusion, R. fruticosus can be efficiently propagated using a modified MS medium.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

GC - Plant growing, crop rotation

OECD FORD branch

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Project

—

Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Proceedings of XI International Rubus nad Ribes Symposium

ISBN

978-946261115-3

ISSN

0567-7572

e-ISSN

—

Number of pages

4

Pages from-to

487-490

Publisher name

International Society for Horticultural Science

Place of publication

Leuven, Belgium

Event location

Asheville, NC

Event date

Jan 1, 2015

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

000391239400074