In vitro multiplication and rooting of two Czech sweet cherry cultivars
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F17%3AN0000036" target="_blank" >RIV/25271121:_____/17:N0000036 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.17660/ActaHortic.2017.1161.49" target="_blank" >http://dx.doi.org/10.17660/ActaHortic.2017.1161.49</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17660/ActaHortic.2017.1161.49" target="_blank" >10.17660/ActaHortic.2017.1161.49</a>
Alternative languages
Result language
angličtina
Original language name
In vitro multiplication and rooting of two Czech sweet cherry cultivars
Original language description
The goal of this study was to identify factors affecting in vitro shoot and root production of two Czech sweet cherry (Prunus avium) cultivars, 'Amid' and 'Kares Frühe' under in vitro culture conditions. This information is required prior to experiments with chemotherapy as a method of sanitation from viruses. The two genotypes were successfully established in vitro using mercuric chloride in a concentration of 0.15% as a sterilization solution. The overall rate of contamination was 16.7%. Six proliferation MS media containing 1, 2 and 4 mg L-1 BAP (6-benzylaminopurine), 0.5 and 1 mg L-1 TDZ (thidiazuron) or 10 mg L-1 2iP (6-(g,g-dimethylallylamino) purine) were tested for effects on proliferation, callus formation and shoot morphology. Multiplication rate values were relatively low and varied between 1.1 and 2.1. BAP at 4 mg L-1 was found to be more effective than TDZ and 2iP for shoot multiplication. Rooting was promoted on MS medium supplied with 1 mg L-1 NAA (naphthaleneacetic acid). Root initiation started within 2 weeks. The root induction was relatively low, 45% for 'Kares Frühe' and 28% for 'Amid'. In conclusion, multiplication and rooting rates were sufficient for in vitro culture establishment, short-term maintenance and in vitro chemotherapy. However, these relatively low rates are not satisfactory for in vitro production of plants on a commercial scale.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
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OECD FORD branch
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)
Result continuities
Project
<a href="/en/project/QJ1210175" target="_blank" >QJ1210175: Research and development of standard methodic procedures of recovering of fruit trees and grapevinesby chemotherapy of in vitro cultures for certification system of healthy status of planting material.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Acta Horticulturae
ISBN
978-94-62611-57-3
ISSN
0567-7572
e-ISSN
2406-6168
Number of pages
5
Pages from-to
303-307
Publisher name
ISHS
Place of publication
Leuven 1, Belgium
Event location
Plasencia, Spain
Event date
Jun 23, 2013
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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