Utilization of molecular methods for evaluation of sweet cherry (Prunus avium L.) genetic resources

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F20%3AN0000054" target="_blank" >RIV/25271121:_____/20:N0000054 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Utilization of molecular methods for evaluation of sweet cherry (Prunus avium L.) genetic resources

Original language description

Sweet cherry is a vegetatively propagated, perennial plant with high level of heterozygosity and ancient breeding history. Sweet cherries are self-incompatible determined by a gametophytic self-incompatibility system (GSI), controlled by a multi-allelic S-locus. Maintenance and conservation of genetic resources are necessary for future breeding programs, growers and fruit production stability. In present, the utilization of DNA molecular genetic methods is the best suitable method for evaluation of genetic variability and S-genotyping within individual accessions. In our work, we used PCR primer combinations for 20 SSR, 2 EST-SSR and 10 universal or specific S-locus markers for analyses of 123 current, old and local sweet cherry cultivars from Czech genetic resources of Research and Breeding Institute of Pomology in Holovousy. In total, 115 polymorphic markers were amplified by SSR markers which we used for hierarchical cluster analysis of genetic variability. Clustering corresponded as with genealogical and geobotanical characteristics of individual accessions as breeding history of several known accessions. We confirmed previous S-genotyping for 72 accessions excepted Drogans Gelbe, Hedelfinger, Erika, Meckenheimer Frühe, Alfa, Huldra, Rivan, Vanda and Winkler’s Frühe. It can be due to mislabelling or mistakes in previous analysis. Totally, we detected S-locus combinations in 19 incompatibility group. The most frequent incompatibility groups were III (S3S4), II (S1S3), IV (S2S3), and VI (S3S6).

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project

<a href="/en/project/QJ1510001" target="_blank" >QJ1510001: Research of sweet cherry germplasm by molecular-genetic methods</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Acta Horticulturae

ISBN

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ISSN

0567-7572

e-ISSN

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Number of pages

8

Pages from-to

441-448

Publisher name

ISHS

Place of publication

Leuven

Event location

Istanbul

Event date

Aug 12, 2018

Type of event by nationality

CST - Celostátní akce

UT code for WoS article

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