A real-time PCR quantitative analysis of the Venturia inaequalis cytb gene G143A mutation and its prevalence in the Czech Republic

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F20%3AN0000114" target="_blank" >RIV/25271121:_____/20:N0000114 - isvavai.cz</a>

Result on the web

<a href="https://www.pubhort.org/ejhs/85/3/4/85_3_4.pdf" target="_blank" >https://www.pubhort.org/ejhs/85/3/4/85_3_4.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17660/eJHS.2020/85.3.4" target="_blank" >10.17660/eJHS.2020/85.3.4</a>

Result language

angličtina

Original language name

A real-time PCR quantitative analysis of the Venturia inaequalis cytb gene G143A mutation and its prevalence in the Czech Republic

Original language description

Venturia inaequalis (Cooke) G. Winter (1875) is a fungal pathogen that causes a disease in apples known as apple scab, which is a major source of economic losses for apple growers. To contain the pathogen, fungicides have been usually applied, often from the strobilurin family. Unfortunately, only two years after the introduction of strobilurin fungicides, resistant V. inaequalis populations appeared, and a substitution of glycine at position 143 with alanine (G143A) in the mitochondrial cytb gene was found to be the mutation responsible. Many methods exist to detect this mutation, but they are generally not suitable for simple routine use, do not provide quantitative data, or are not sensitive enough. Moreover, the quality and quantity of isolated DNA from fungi may also be an issue due to the frequent presence of PCR inhibitors. A new real-time PCR-based assay was therefore designed that can detect 0.1% of the G143A mutation in a wild type background and vice versa; in parallel, DNA isolation was optimized, using 10 million conidia in a suspension prepared from leaves harvested in orchards instead of the commonly used scab mycelia. The assay was then used for screening of the resistant V. inaequalis in orchards. The G143A mutation was present in all conventional and organic orchards tested, and in the vast majority it was present at nearly or at 100%, indicating that the mutation does not likely affect the fitness of V. inaequalis and the mutation persists over time. Furthermore, the G143A mutation (average 2.3%) was also observed in V. inaequalis isolates harvested from alley trees along roads, implying that the mutation also occurs naturally at low levels.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

40100 - Agriculture, Forestry, and Fisheries

Project

<a href="/en/project/QJ1510353" target="_blank" >QJ1510353: Increasing of the effectiveness of apple trees protection against apple scab</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

European Journal of Horticultural Science

ISSN

1611-4426

e-ISSN

—

Volume of the periodical

85

Issue of the periodical within the volume

3

Country of publishing house

DE - GERMANY

Number of pages

7

Pages from-to

169-175

UT code for WoS article

000543446600004

EID of the result in the Scopus database

2-s2.0-85086832193