A rapid real-time PCR assay for detecting columnar growth in apples (Malus × domestica Borkh.)

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F20%3AN0000127" target="_blank" >RIV/25271121:_____/20:N0000127 - isvavai.cz</a>
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/abs/10.1111/pbr.12861" target="_blank" >https://onlinelibrary.wiley.com/doi/abs/10.1111/pbr.12861</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/pbr.12861" target="_blank" >10.1111/pbr.12861</a>

Result language
angličtina
Original language name
A rapid real-time PCR assay for detecting columnar growth in apples (Malus × domestica Borkh.)
Original language description
Columnar growth in apples can have considerable positive consequences for orchard maintenance, but the lack of columnar cultivars producing fruits with desired market quality prevents their broader utilization in orchards. New crossings focused on their breeding are therefore conducted throughout the world every year. Unfortunately, the reliable discrimination between columnar growth and normal phenotype is difficult until seedlings are 2-3 years of age. However, molecular marker assisted selection (MAS) can be used in seedlings several weeks old providing immense help in breeding programs focused on columnar apple production. Here, we describe a rapid and reliable method for the detection of both wild type (WT) and columnar (Co) alleles originating from ‘McIntosh Wijcik’ in a single reaction using a real-time PCR allelic discrimination approach. Our assay was tested in 130 genotypes (80 normal and 50 columnar habit) from various crossings and their parents, and in all cases, genotype corresponded to phenotype. The assay is thus suitable for laboratories interested in high-throughput MAS for columnar tree habit breeding.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project
<a href="/en/project/LO1608" target="_blank" >LO1608: Research Pomological Centre</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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