Use of X-ray-induced mutation in sweet cherry breeding

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F23%3AN0000086" target="_blank" >RIV/25271121:_____/23:N0000086 - isvavai.cz</a>

Result on the web

<a href="https://www.actahort.org/books/1362/1362_26.htm" target="_blank" >https://www.actahort.org/books/1362/1362_26.htm</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Use of X-ray-induced mutation in sweet cherry breeding

Original language description

An article of Acta Horticulturae symposium proceedings (ISHS Acta Horticulturae 1362: XXXI International Horticultural Congress (IHC2022): International Symposium on Breeding and Effective Use of Biotechnology and Molecular Tools in Horticultural Crops) Sweet cherry (Prunus avium L.) breeding programs produce commercial cuiltivars primarily using traditional breeding methods. Induced mutations and molecular biology methods represent a tool for acceleration and refining the breeding process. An increasingly desired feature is the self-pollination of sweet cherries. They are primarily self-incompatible, but some gene mutations can enable the self-compatibility. We induced the mutation of genes leading to self-pollination at the RBIP Holovousy in the Czech Republic. Branches with generative buds in the meiotic phase of pollen grains of sweet cherry cultivars ‘Tamara’, ‘Irena’ and ‘Burlat’ were irradiated with X-rays at a dose of 20 Gy. In 3 years, 493 sweet cherry embryos were sown, out of which 34 individuals were raised. They were then subjected to fragment analyses to determine the combination of S-alleles. An individual with a combination of S-alleles: mutated S4’S6 was identified in the genotypes. The initial combination of S-alleles was S4S6. In this individual, a mutation in the S4 allele is expected and will be verified by genotyping using SSR markers.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project

<a href="/en/project/QK1910290" target="_blank" >QK1910290: Development and application of molecular genetic methods for the rationalization of sweet cherry breeding practices (Prunus avium L.)</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

ISHS Acta Horticulturae 1362 XXXI International Horticultural Congress (IHC2022): International Symposium on Breeding and Effective Use of Biotechnology and Molecular Tools in Horticultural Crops

ISBN

978-94-62613-61-4

ISSN

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e-ISSN

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Number of pages

4

Pages from-to

195-198

Publisher name

ISHS

Place of publication

Leuven, Belgium

Event location

Angers, Francie

Event date

Aug 14, 2022

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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