Clarithromycin Suppresses Apple Proliferation Phytoplasma in Explant Cultures

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F23%3AN0000136" target="_blank" >RIV/25271121:_____/23:N0000136 - isvavai.cz</a>
Result on the web
<a href="https://www.mdpi.com/2223-7747/12/22/3820" target="_blank" >https://www.mdpi.com/2223-7747/12/22/3820</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/plants12223820" target="_blank" >10.3390/plants12223820</a>

Result language
angličtina
Original language name
Clarithromycin Suppresses Apple Proliferation Phytoplasma in Explant Cultures
Original language description
Apple proliferation, caused by ‘Candidatus Phytoplasma mali’, is one of the most important economic threats in the field of apple production. Especially at a young age, infected trees can be affected by excessive bud proliferation and general decline. The fruit quality is also significantly reduced by this disease. To investigate treatment options, we applied a clarithromycin chemotherapy to infected in vitro cultures of ‘Golden Delicious’. With increasing concentrations of clarithromycin in the media, the phytoplasma load decreased rapidly after one month of treatment, but phytotoxicity led to a pronounced mortality at 40 mg/L, which was the highest dose used in our experiment. Out of 45 initial explants, we obtained one negative mericlone and two mericlones with a concentration of phytoplasma DNA at the detection limit of PCR. The culture propagated from the mericlone that tested negative remained phytoplasma-free after 18 months of subculturing. Our results suggest the applicability of macrolide antibiotics against phytoplasma infections in vitro; however, it might be challenging to find the threshold zone where the concentration is sufficient for pathogen elimination, but not lethal for the plant material of different cultivars.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
40401 - Agricultural biotechnology and food biotechnology

Project
<a href="/en/project/QK21020395" target="_blank" >QK21020395: Production of phytoplasmas free nursery material and its protection against reinfection</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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