In Vitro Propagation of Sweet Rowanberry Cultivar Discolor as an Alternative Fruit Crop Resource

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F25%3AN0000108" target="_blank" >RIV/25271121:_____/25:N0000108 - isvavai.cz</a>

Result on the web

<a href="https://www.mdpi.com/2073-4395/15/12/2812" target="_blank" >https://www.mdpi.com/2073-4395/15/12/2812</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/agronomy15122812" target="_blank" >10.3390/agronomy15122812</a>

Result language

angličtina

Original language name

In Vitro Propagation of Sweet Rowanberry Cultivar Discolor as an Alternative Fruit Crop Resource

Original language description

Developing reliable in vitro propagation methods for rowanberry genotypes is essential for their use in breeding and horticultural programs. While different rowanberry species and cultivars are primarily valued for their ornamental and forestry properties, poor seed germination and the low success rate of conventional vegetative techniques constrain their propagation. Micropropagation offers a practical approach to obtaining uniform, disease-free plant material for selection, hybridization, and the subsequent horticultural use of particular valuable genotypes. Shoot multiplication of a prospective sweet rowanberry cultivar ‘Discolor’ was studied on a Murashige and Skoog (MS) medium supplemented with 6-benzylaminopurine (BAP) at concentrations of 1, 2, and 4 mg L−1, thidiazuron (TDZ) at concentrations of 0.5 and 1 mg L−1, and 6-(γ,γ-dimethylallylamino)purine (2iP) at a concentration of 10 mg L−1. Root induction was evaluated on a half-strength MS medium (50% MS) supplemented with 1 mg L−1 of naphthaleneacetic acid (NAA), indole-3-butyric acid (IBA), or indole-3-acetic acid (IAA). TDZ at 1 mg L−1 yielded the highest multiplication coefficient. However, media with TDZ at a lower concentration (0.5 mg L−1) or BAP (2–4 mg L−1) provided the best balance between proliferation rate and shoot quality. These media promoted the growth of vigorous, well-elongated shoots with minimal callus formation. In contrast, the phytohormone 2iP did not elicit physiological response in the in vitro multiplication of explants. The best rooting results were obtained using a 50% MS medium supplemented with 1 mg L−1 IAA, which provided the highest rooting percentage and root quality. IBA produced slightly lower, though comparable, results, while NAA resulted in weak, sporadic root formation. The established protocol enables the efficient in vitro propagation of the studied cultivar. This system supports its application in breeding and fruit production programs, as well as in maintaining valuable genetic resources within the genus Sorbus.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40401 - Agricultural biotechnology and food biotechnology

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Agronomy

ISSN

2073-4395

e-ISSN

—

Volume of the periodical

15

Issue of the periodical within the volume

12

Country of publishing house

CH - SWITZERLAND

Number of pages

14

Pages from-to

2812

UT code for WoS article

001646083600001

EID of the result in the Scopus database

2-s2.0-105025923346