Species-specific detection of Bipolaris sorokiniana from wheat and barley tissues

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25328859%3A_____%2F10%3A%230000504" target="_blank" >RIV/25328859:_____/10:#0000504 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Species-specific detection of Bipolaris sorokiniana from wheat and barley tissues

Original language description

Cochliobolus sativus (anamorph: Bipolaris sorokiniana) is a fungal pathogen that can cause yield limiting diseases of barley and wheat. A PCR-based diagnostic assay was developed to detect B. sorokiniana in barley and wheat tissues using the pathogen-specific primers COSA_F/R derived from the melanin biosynthesis pathway Brn1 locus. Specificity was tested against more than 30 fungal organisms associated with barley and wheat. Limit of detection was determined to be 0.001 ng for pure fungal DNA. The species-specific primers were capable of detecting the pathogen in planta, specifically from infected barley leaves and wheat roots and stem bases. (C) 2010 Published by Elsevier Ltd.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GF - Diseases, pests, weeds and plant protection

OECD FORD branch

—

Project

<a href="/en/project/QH71213" target="_blank" >QH71213: Creation of new spring barley genotypes characterized by complex resistance to leaf and spike diseases</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Crop Protection

ISSN

0261-2194

e-ISSN

—

Volume of the periodical

29

Issue of the periodical within the volume

11

Country of publishing house

GB - UNITED KINGDOM

Number of pages

6

Pages from-to

—

UT code for WoS article

000282560400015

EID of the result in the Scopus database

—