Reaction of winter wheat cultivars to eyespot assessed visually and by real-time PCR

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25328859%3A_____%2F20%3AN0000003" target="_blank" >RIV/25328859:_____/20:N0000003 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15310/20:73603310 RIV/00027006:_____/20:10149297
Result on the web
<a href="https://www.agriculturejournals.cz/publicFiles/52_2019-CJGPB.pdf" target="_blank" >https://www.agriculturejournals.cz/publicFiles/52_2019-CJGPB.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17221/52/2019-CJGPB" target="_blank" >10.17221/52/2019-CJGPB</a>

Result language
angličtina
Original language name
Reaction of winter wheat cultivars to eyespot assessed visually and by real-time PCR
Original language description
The reaction of twelve winter wheat cultivars frequently grown in the Czech Republic and twenty-five new breeding lines to inoculation with Oculimacula yallundae and Oculimacula acuformis was evaluated in small plot trials from 2017-2018. The assessment was carried out visually by symptoms and by a quantitative real- time polymerase chain reaction (qPCR). The aims of the study were to compare the results of both methods, to evaluate the effect of the resistance gene Pch1 to eyespot, and to select new breeding lines resistant to eyespot. The relationship between the eyespot symptoms and the pathogen DNA content in plant tissues followed a moderate linear regression. Low levels of eyespot were observed in the cultivars/lines possessing the resistance gene Pch1 (Annie, Hermann, Rebell, SG-S1215-14, SG-S1825-14, SG-S791-13) and also in the line SG-SU630-15. The qPCR method was able to detect low levels of the pathogens in the plant tissue and to distinguish two eyespot pathogens. O. acuformis was detected in very low concentrations in the inoculated plants compared with O. yallundae. The eyespot infection rate was significantly higher in 2017 than in the next agricultural season due to extremely dry and warm spring weather in 2018.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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