EliGene® Colorectum NGS Kit
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26258412%3A_____%2F19%3AN0000002" target="_blank" >RIV/26258412:_____/19:N0000002 - isvavai.cz</a>
Result on the web
<a href="https://www.eligene.com/eligene-colorectum.html" target="_blank" >https://www.eligene.com/eligene-colorectum.html</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
EliGene® Colorectum NGS Kit
Original language description
EliGene® Colorectum NGS kit is intended for multiplex PCR of target regions of KRAS (exons 2,3 and 4), NRAS (exons 2,3 and 4) and BRAF genes (exon 15) allowing detection of polymorphisms included in the amplified regions.
Czech name
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Czech description
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Classification
Type
G<sub>prot</sub> - Prototype
CEP classification
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OECD FORD branch
30403 - Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions [pharmacogenomics, gene-based therapeutics])
Result continuities
Project
<a href="/en/project/EG15_018%2F0004701" target="_blank" >EG15_018/0004701: Industrial research and development of new DNA technology for human diagnostics: next generation sequencing and microRNA</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
C - Předmět řešení projektu podléhá obchodnímu tajemství (§ 504 Občanského zákoníku), ale název projektu, cíle projektu a u ukončeného nebo zastaveného projektu zhodnocení výsledku řešení projektu (údaje P03, P04, P15, P19, P29, PN8) dodané do CEP, jsou upraveny tak, aby byly zveřejnitelné.
Data specific for result type
Internal product ID
90066-NGS
Numerical identification
90066-NGS
Technical parameters
EliGene® Colorectum NGS kit is intended for multiplex PCR of target regions of KRAS (exons 2,3 and 4), NRAS (exons 2,3 and 4) and BRAF genes (exon 15) allowing detection of polymorphisms included in the amplified regions. EliGene® Colorectum NGS kits enable multiplex PCR amplification of all required target regions of the gene(s) of interest. The recommended amount of DNA for each multiplex PCR reaction is between 20 and 100 ng of DNA derived from formalin-fixed paraffin-embedded (FFPE) material or from blood. Next, the resulting amplicons are barcoded with MID sequences, and mixed per sample. This sample is quantified and sequenced using a NGS instrument Illumina for massively parallel sequencing (MPS) according to the manufacturer’s instructions. The resulting sequence reads are subsequently analyzed by comparison with the reference sequence of the targeted gene(s) to identify variant positions.
Economical parameters
Prototyp přispěl k výrobě soupravy, která se podílela na ročním obratu společnosti ve výši 1,81 %.
Application category by cost
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Owner IČO
26258412
Owner name
ELISABETH PHARMACON, spol. s r.o.
Owner country
CZ - CZECH REPUBLIC
Usage type
V - Výsledek je využíván vlastníkem
Licence fee requirement
A - Poskytovatel licence na výsledek požaduje licenční poplatek
Web page
https://www.eligene.com/eligene-colorectum.html