Assessment of genetic and epigenetic stability in long-term in vitro shoot culture of pea (Pisum sativum L.)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26784246%3A_____%2F07%3A%230000045" target="_blank" >RIV/26784246:_____/07:#0000045 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Assessment of genetic and epigenetic stability in long-term in vitro shoot culture of pea (Pisum sativum L.)

Original language description

We have used several molecular methods to assess the genetic stability of long-term propagated (24 years) multiple shoot in vitro culture of pea (Pisum sativum L.). We focused on assessing the stability of repetitive sequences, such as simple sequence repeats (SSR) and retrotransposons, both comprising a large part of genome. No differences were found between controls and long-term or newly established in vitro (one subculture cycle) samples were investigated by the SSR, inter-repeats (ISSR) or inter-retrotransposon amplified polymorphism (IRAP) method. However, the more global amplified fragment length polymorphism (AFLP) and the methylation sensitive MSAP methods detected 11 and 18% polymorphism. Some evidence of retrotransposon re-arrangement was observed by sequence-specific amplification polymorphism. Based on these results, multiple shoot culture of pea maintained over a long period may be considered as a true to type multiplication method of the original genotype.

Czech name

Studium genetické a epigenetické stability dlouhodobé in vitro kultury hrachu (Pisum sativum L.)

Czech description

Pomocí molekulárních metod byla studována genetické a epigenetická stabilita dlouhodobé in vitro kultury vzrostných prýtů hrachu. Mikrosatelitními a retrotransposonovými markery nebyla zjištěny žádné významné změny. Tyto byly detekovány pouze pomocí metylačně citlivé analýzy (MSAP), kdy byl zjištěn 11 a 18% polymorfismus. Tyto změny v metylaci genomové DNA byly potvrzeny pomocí měření celkového zastoupení metyl-cytosinu metdou HPCE.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2007

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Cell Reports

ISSN

0721-7714

e-ISSN

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Volume of the periodical

26

Issue of the periodical within the volume

11

Country of publishing house

DE - GERMANY

Number of pages

14

Pages from-to

1985-1998

UT code for WoS article

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EID of the result in the Scopus database

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