Resolving browning during the establishment of explant cultures in Vicia faba L. for genetic transformation.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26784246%3A_____%2F13%3A%230000791" target="_blank" >RIV/26784246:_____/13:#0000791 - isvavai.cz</a>

Result on the web

<a href="http://acta.mendelu.cz/61/5/1279/" target="_blank" >http://acta.mendelu.cz/61/5/1279/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.11118/actaun201361051279" target="_blank" >10.11118/actaun201361051279</a>

Result language

angličtina

Original language name

Resolving browning during the establishment of explant cultures in Vicia faba L. for genetic transformation.

Original language description

Optimisation of two in vitro regeneration systems for low-tannine cultivars of faba bean based on culturing of shoot apices and cotyledonary nodes were provided by usage of various antioxidants - ascorbic acid, citric acid, glutathione and activated charcoal. In subsequent testing, the combined effects of antioxidants with transformation co-cultivation compounds acetosyringone and L-cysteine was studied. The application of antioxidants lead to decreased caullogenesis, citric acids treatments (50 mg.l-1)dramatically decreased necrotic response of explants. However, citric acid, used together with ascorbic acid completely inhibited shoot growth in shoot apex cultures. Glutathion evoked hyperhydricity of explants. Activated charcoal induced rooting on media which are commonly used for shoot proliferation. Combination of acetosyringone with antioxidants did not negatively influenced shoot proliferation, except of variant with ascorbic acid. Citric acid worked as the best and universal ant

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EI - Biotechnology and bionics

OECD FORD branch

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Project

<a href="/en/project/QI91A229" target="_blank" >QI91A229: Application of conventional and molecular genetic approaches for the development of grain legumes resistant to viral and fungal pathogens and insect pests.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis

ISSN

1211-8516

e-ISSN

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Volume of the periodical

61

Issue of the periodical within the volume

5

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

10

Pages from-to

1279-1288

UT code for WoS article

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EID of the result in the Scopus database

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