Determination of T-2 and HT-2 Toxins in Seed of Milk Thistle [<i>Silybum marianum</i> (L.) Gaertn.] Using Immunoaffinity Column by UPLC-MS/MS

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26784246%3A_____%2F22%3AN0000064" target="_blank" >RIV/26784246:_____/22:N0000064 - isvavai.cz</a>

Result on the web

<a href="https://www.mdpi.com/2072-6651/14/4/258" target="_blank" >https://www.mdpi.com/2072-6651/14/4/258</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/toxins14040258" target="_blank" >10.3390/toxins14040258</a>

Result language

angličtina

Original language name

Determination of T-2 and HT-2 Toxins in Seed of Milk Thistle [<i>Silybum marianum</i> (L.) Gaertn.] Using Immunoaffinity Column by UPLC-MS/MS

Original language description

Milk thistle [Silybum marianum (L.) Gaertn.] achieved a significant increase in interest over the past few years from local and foreign pharmaceutical corporations. The silymarin complex of constituents extracted from milk thistle achenes provides compelling health benefits primarily thanks to antioxidant activities and hepatoprotective effects. However, consuming mycotoxin-contaminated plant material can cause immunosuppression and hepatotoxic problems. The aim of this study was to develop and validate a method for the determination of mycotoxin content in milk thistle. Fusarium toxins as T-2 and HT-2 toxins in grown milk thistle harvested from a breeding station in the Czech Republic during 2020–2021 were studied. The analysis of T-2 and HT-2 toxins was performed by UPLC-MS/MS after immunoaffinity columns EASI-EXTRACT® T-2 & HT-2 clean up. All analysed samples of milk thistle were contaminated with T-2 toxin and HT-2 toxin. The content of T-2 toxin in the samples from 2020 was in the range of 122.7–290.2 µg/kg and HT-2 toxin 157.0–319.0 µg/kg. In 2021, the content of T-2 toxin was in the range of 28.8–69.9 µg/kg and HT-2 toxin was 24.2–75.4 µg/kg. The results show that the climatic conditions of the year of harvesting have a highly statistically significant effect on the content of T-2 and HT-2 toxins in milk thistle.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10700 - Other natural sciences

Project

<a href="/en/project/QK1910302" target="_blank" >QK1910302: Processing of waste products obtained during oil extraction from oil crop seeds on new commodities with nutritional and health benefits</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

TOXINS

ISSN

2072-6651

e-ISSN

2072-6651

Volume of the periodical

14

Issue of the periodical within the volume

4

Country of publishing house

CH - SWITZERLAND

Number of pages

12

Pages from-to

258

UT code for WoS article

000786002900001

EID of the result in the Scopus database

2-s2.0-85128614722