New Low Morphine Opium Poppy Genotype Obtained by TILLING Approach

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26784246%3A_____%2F23%3AN0000002" target="_blank" >RIV/26784246:_____/23:N0000002 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12220/23:43906658 RIV/26791251:_____/23:N0000056
Result on the web
<a href="https://www.mdpi.com/2223-7747/12/5/1077" target="_blank" >https://www.mdpi.com/2223-7747/12/5/1077</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/plants12051077" target="_blank" >10.3390/plants12051077</a>

Result language
angličtina
Original language name
New Low Morphine Opium Poppy Genotype Obtained by TILLING Approach
Original language description
The opium poppy’s ability to produce various alkaloids is both useful and problematic. Breeding of new varieties with varying alkaloid content is therefore an important task. In this paper, the breeding technology of new low morphine poppy genotypes, based on a combination of a TILLING approach and single-molecule real-time NGS sequencing, is presented. Verification of the mutants in the TILLING population was obtained using RT-PCR and HPLC methods. Only three of the single-copy genes of the morphine pathway among the eleven genes were used for the identification of mutant genotypes. Point mutations were obtained only in one gene (CNMT) while an insertion was obtained in the other (SalAT). Only a few expected transition SNPs from G:C to A:T were obtained. In the low morphine mutant genotype, the production of morphine was decreased to 0.1% from 1.4% in the original variety. A comprehensive description of the breeding process, a basic characterization of the main alkaloid content, and a gene expression profile for the main alkaloid-producing genes is provided. Difficulties with the TILLING approach are also described and discussed.
Czech name
—
Czech description
—

Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming

Project
<a href="/en/project/QK1810391" target="_blank" >QK1810391: Utilization of genomic and transcriptomic approaches to create genetic resources and breeding materials of poppy with specific traits</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)