Early microvascular changes with loss of the glycocalyx in children with type 1 diabetes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F27283933%3A_____%2F14%3AN0000026" target="_blank" >RIV/27283933:_____/14:N0000026 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.jpeds.2013.11.016" target="_blank" >http://dx.doi.org/10.1016/j.jpeds.2013.11.016</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jpeds.2013.11.016" target="_blank" >10.1016/j.jpeds.2013.11.016</a>

Result language

angličtina

Original language name

Early microvascular changes with loss of the glycocalyx in children with type 1 diabetes

Original language description

OBJECTIVE: To evaluate the microcirculation of children with type 1 diabetes mellitus who demonstrate no clinical signs of diabetic microangiopathy for the presence of microvascular alterations and glycocalyx perturbation. STUDY DESIGN: Images of sublingual vessels were obtained in 14 children with diabetes (ages 12.8 ± 2.8 years, diabetes duration 6.7 ± 4.3 years) and 14 control patients (ages 11.8 ± 2.8 years) by the use of sidestream dark field imaging and analyzed for total vessel density, vessel surface coverage, vessel diameter distribution, mean flow index, and glycocalyx thickness. Wilcoxon rank sum test and Pearson correlation were used for statistical analysis. RESULTS: We observed profound microcirculatory changes in children with diabetes compared with control patients, with a significant reduction of glycocalyx thickness (0.38 μm vs 0.60 μm; P = .013), which was inversely correlated with blood glucose levels (r = -0.55; P = .003). Furthermore, the percentage of large vessels (>20 μm diameter) was significantly increased (11% vs 6%; P = .023) at the expense of capillaries (<10 μm diameter) with consequent increase in total vessel surface coverage (30% vs 26.0%; P = .041). No differences were seen in total vessel density and mean flow index. CONCLUSIONS: Microvascular alterations, including changes in microvessel distribution and loss of the glycocalyx, can be detected in children with type 1 diabetes mellitus before clinically apparent vascular complications. Our results disclose the glycocalyx as a possible monitoring measurement for earlier detection of diabetic microangiopathy and may provide a basis for new therapeutic strategies aiming at protection or restoration of the glycocalyx.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FG - Paediatrics

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

The Journal of Pediatrics

ISSN

0022-3476

e-ISSN

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Volume of the periodical

164

Issue of the periodical within the volume

3

Country of publishing house

US - UNITED STATES

Number of pages

6

Pages from-to

584-9

UT code for WoS article

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EID of the result in the Scopus database

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