Dysregulated expression of lncRNAs in glioblastoma multiforme and their association with overall survival

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F27661989%3A_____%2F19%3AN0000011" target="_blank" >RIV/27661989:_____/19:N0000011 - isvavai.cz</a>

Result on the web

<a href="https://aacrjournals.org/cancerres/article/79/13_Supplement/3575/635487/Abstract-3575-Dysregulated-expression-of-lncRNAs" target="_blank" >https://aacrjournals.org/cancerres/article/79/13_Supplement/3575/635487/Abstract-3575-Dysregulated-expression-of-lncRNAs</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1158/1538-7445.AM2019-3575" target="_blank" >10.1158/1538-7445.AM2019-3575</a>

Result language

angličtina

Original language name

Dysregulated expression of lncRNAs in glioblastoma multiforme and their association with overall survival

Original language description

Introduction: Glioblastoma multiforme (GBM) is the most frequent primary brain malignancy of astrocytic origin. The prognosis remains very poor with the median overall survival (OS) being between 12 and 16 months from diagnosis despite early use of conventional medical therapy. Identifying new therapeutic targets, as well as prognostic and predictive biomarkers for accurate stratification of patients is therefore of utmost importance. Long non-coding RNAs (lncRNAs) are regulators of gene expression having critical impact on both physiological processes and the molecular pathology of GBM, indicating their potential as biomarkers and therapeutic targets. Material and Methods: Our study included 219 GBM patients and 29 patients with non-dominant anterior temporal cortexes resected during surgery for intractable epilepsy. Informed consent approved by the local Ethical Commission was obtained from each patient. RNA (RIN > 8) from 77 specimens was used for next-generation RNA sequencing (RNAseq). rRNA depletion and cDNA library preparation were done with RiboCop rRNA Depletion Kit V1.2 (Lexogen) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB), respectively. RNAseq was performed using NextSeq 500 High Output Kit and NextSeq 500 instrument (both Illumina). 8,414 lncRNAs and their sequential variants with non-zero RPKM at least in one sample were statistically evaluated. The alignment and target counts were performed with CLC genomic workbench. Selected significantly dysregulated lncRNAs between GBM and non-tumor controls were analyzed in a larger cohort of 188 specimens by qRT-PCR and the expression data normalized to PPIA was then evaluated by Mann-Whitney U test.

Czech name

—

Czech description

—

Type

J_ost - Miscellaneous article in a specialist periodical

CEP classification

—

OECD FORD branch

30200 - Clinical medicine

Project

<a href="/en/project/NV18-03-00398" target="_blank" >NV18-03-00398: Extension of established prognostic scores in brain metastases by microRNA profiling to tailor the post-operative personalized care</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cancer Research

ISSN

0008-5472

e-ISSN

1538-7445

Volume of the periodical

79

Issue of the periodical within the volume

13 Supplement S

Country of publishing house

US - UNITED STATES

Number of pages

1

Pages from-to

—

UT code for WoS article

000488279402481

EID of the result in the Scopus database

—