Preservation of urine specimens for metabolic evaluation of recurrent urinary stone formers
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F27661989%3A_____%2F25%3A10002847" target="_blank" >RIV/27661989:_____/25:10002847 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11150/25:10488697
Result on the web
<a href="https://www.degruyterbrill.com/document/doi/10.1515/cclm-2024-0773/html?srsltid=AfmBOoodGbOqkCpJ0bowC-gZ6gBF_hx5e36rFC6fTQuSbL0F0NCleD6M" target="_blank" >https://www.degruyterbrill.com/document/doi/10.1515/cclm-2024-0773/html?srsltid=AfmBOoodGbOqkCpJ0bowC-gZ6gBF_hx5e36rFC6fTQuSbL0F0NCleD6M</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1515/cclm-2024-0773" target="_blank" >10.1515/cclm-2024-0773</a>
Alternative languages
Result language
angličtina
Original language name
Preservation of urine specimens for metabolic evaluation of recurrent urinary stone formers
Original language description
Objectives Stability of concentrations of urinary stone-related metabolites was analyzed from samples of recurrent urinary stone formers to assess necessity and effectiveness of urine acidification during collection and storage.Methods First-morning urine was collected from 20 adult calcium-stone forming patients at Tomas Bata Hospital in the Czech Republic. Urine samples were analyzed for calcium, magnesium, inorganic phosphate, uric acid, sodium, potassium, chloride, citrate, oxalate, and urine particles. The single-voided specimens were collected without acidification, after which they were divided into three groups for storage: samples without acidification ("NON"), acidification before storage ("PRE"), or acidification after storage ("POST"). The analyses were conducted on the day of arrival (day 0, "baseline"), or after storage for 2 or 7 days at room temperature. The maximum permissible difference (MPD) was defined as +/- 20 % from the baseline.Results The urine concentrations of all stone-related metabolites remained within the 20 % MPD limits in NON and POST samples after 2 days, except for calcium in NON sample of one patient, and oxalate of three patients and citrate of one patient in POST samples. In PRE samples, stability failed in urine samples for oxalate of three patients, and for uric acid of four patients after 2 days. Failures in stability often correlated with high baseline concentrations of those metabolites in urine.Conclusions Detailed procedures are needed to collect urine specimens for analysis of urinary stone-related metabolites, considering both patient safety and stability of those metabolites. We recommend specific preservation steps.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
20602 - Medical laboratory technology (including laboratory samples analysis; diagnostic technologies) (Biomaterials to be 2.9 [physical characteristics of living material as related to medical implants, devices, sensors])
Result continuities
Project
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Continuities
N - Vyzkumna aktivita podporovana z neverejnych zdroju
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Clinical Chemistry and Laboratory Medicine
ISSN
1434-6621
e-ISSN
1437-4331
Volume of the periodical
63
Issue of the periodical within the volume
1
Country of publishing house
DE - GERMANY
Number of pages
10
Pages from-to
129-138
UT code for WoS article
001278957200001
EID of the result in the Scopus database
2-s2.0-85200157841