Clonal relatedness and emerging linezolid resistance in Clostridioides difficile ribotypes 001 and 176 in the Czech Republic
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F27661989%3A_____%2F26%3A10003560" target="_blank" >RIV/27661989:_____/26:10003560 - isvavai.cz</a>
Alternative codes found
RIV/00064211:_____/26:W0000092 RIV/00064190:_____/26:10001450
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S1201971226000792" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1201971226000792</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ijid.2026.108444" target="_blank" >10.1016/j.ijid.2026.108444</a>
Alternative languages
Result language
angličtina
Original language name
Clonal relatedness and emerging linezolid resistance in Clostridioides difficile ribotypes 001 and 176 in the Czech Republic
Original language description
Background and aims: Clostridioides difficile ribotypes (RTs) 001 and 176 dominate the epidemiology of C. difficile infection (CDI) in the Czech Republic. We applied whole-genome sequencing (WGS) and broad-range antimicrobial susceptibility testing (AST) to provide detailed characterization of these lineages. Methods: Between October and December 2019, 22 hospitals participated. CDI isolates were characterized by PCR ribotyping, toxin gene detection and AST to metronidazole, vancomycin and moxifloxacin. WGS and broad-range AST were performed on selected RT001 and RT176 isolates. Results: The mean CDI incidence was 5.1 cases/10,000 patient-days. Isolates and epidemiological data were available for 495/524 (94.5%) CDIs. RT001 ( n = 166, 33.5%), RT014 ( n = 59, 11.9%) RT176 ( n = 51, 10.3%) were the most prevalent. Among 21 RT001 isolates, wgMLST (3745 loci) revealed 0-120 allele differences and 21 genomic inserts, eight of which carried antimicrobial resistance (AMR) genes, including cfrB, which encodes linezolid resistance. In 13 RT176 isolates, wgMLST (3298 loci) showed 0-9 allele differences and 11 inserts, eight with AMR genes. The wgMLST confirmed clonal relatedness of RT001 and RT176 isolates from different hospitals (0-3 allelic differences), yet with variation in acquired AMR gene content. Differences between genotype and expected phenotype were observed in PnimBG and metronidazole, cfrE and linezolid and tetgenes and tetracycline. Conclusion: RT001 and RT176 predominate in the Czech Republic, and WGS confirmed their inter-hospital clonal relatedness. Importantly, the emergence of linezolid-resistant RT001 strains was detected in nine hospitals. The inclusion of AMR genes in genetic relatedness analysis showed higher discriminatory power compared to cgMLST or wgMLST alone. (c) 2026 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30230 - Other clinical medicine subjects
Result continuities
Project
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Continuities
O - Projekt operacniho programu
Others
Publication year
2026
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Infectious Diseases
ISSN
1201-9712
e-ISSN
1878-3511
Volume of the periodical
165
Issue of the periodical within the volume
April
Country of publishing house
GB - UNITED KINGDOM
Number of pages
9
Pages from-to
nestránkováno
UT code for WoS article
001705165000001
EID of the result in the Scopus database
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