Resazurin assay for assessment of antimicrobial properties of electrospun nanofiber filtration membranes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F28778758%3A_____%2F19%3AN0000006" target="_blank" >RIV/28778758:_____/19:N0000006 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14310/19:00107010 RIV/46504711:_____/19:N0000035 RIV/61989592:15310/19:73597989 RIV/70883521:28610/19:63523898 RIV/62157124:16270/19:43877445

Result on the web

<a href="https://amb-express.springeropen.com/articles/10.1186/s13568-019-0909-z" target="_blank" >https://amb-express.springeropen.com/articles/10.1186/s13568-019-0909-z</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s13568-019-0909-z" target="_blank" >10.1186/s13568-019-0909-z</a>

Result language

angličtina

Original language name

Resazurin assay for assessment of antimicrobial properties of electrospun nanofiber filtration membranes

Original language description

We developed a simple and fast microplate assay for evaluation of the antimicrobial activity of electrospun nanofiber filtration membranes or similar porous materials for water treatment technologies. Resazurin (alamarBlue®) was used as an indicator of the amount of viable experimental microorganisms Gram-negative Escherichia coli, Gram-positive Enterococcus faecalis, and natural wastewater treatment plant effluent bacteria. A bacterial inoculum of concentration 1–3 × 105 CFU mL−1 was pipetted onto the surface of assessed both functionalized and respective control membranes and incubated in 12-well plates for 4 h at 37 °C. Kinetics of resazurin metabolization, i.e. its reduction to fluorescent resorufin, was evaluated fluorimetrically (λex520/λem590 nm). A number of viable bacteria on the membranes expressed as CFU mL−1 was calculated from the kinetic curves by using calibration curves that were constructed for both experimental bacterial species. Antimicrobial activities of the membranes were evaluated by either resazurin assay or modified ISO 20743 plate count assay. Results of both assays showed the significant antimicrobial activity of membranes functionalized with silver nanoparticles for both bacterial species and wastewater treatment plant effluent bacteria as well (log CFU reduction compared to control membrane > 4), while membranes containing specific quaternary ammonium salts were inefficient (log CFU reduction < 1). The suitability of resazurin microplate assay for testing nanofiber filtration membranes and analogous matrices has proven to be a faster and less demanding alternative to the traditionally used approach providing comparable results.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

20801 - Environmental biotechnology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

AMB Express

ISSN

2191-0855

e-ISSN

2191-0855

Volume of the periodical

9

Issue of the periodical within the volume

13 November 2019

Country of publishing house

GB - UNITED KINGDOM

Number of pages

1

Pages from-to

183-183

UT code for WoS article

000497513200002

EID of the result in the Scopus database

2-s2.0-85075142983