Optimization of Extraction of Cannabinoids and Flavonoids Substances from Influence Cannabis sp. and their Biological Activities before and after Decarboxylation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F28973852%3A_____%2F22%3AN0000004" target="_blank" >RIV/28973852:_____/22:N0000004 - isvavai.cz</a>
Result on the web
<a href="https://thescipub.com/abstract/10.3844/ajassp.2022.68.77" target="_blank" >https://thescipub.com/abstract/10.3844/ajassp.2022.68.77</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3844/ajassp.2022.68.77" target="_blank" >10.3844/ajassp.2022.68.77</a>
Alternative languages
Result language
angličtina
Original language name
Optimization of Extraction of Cannabinoids and Flavonoids Substances from Influence Cannabis sp. and their Biological Activities before and after Decarboxylation
Original language description
Inflorescence of eight different varieties of Cannabis sp. (3 hemp varieties and 5 medical cannabis varieties) were subjected to extraction with n-hexane or ethanol as representatives of non-polar and polar solvents. Crude extracts were either used directly or after decarboxylation of cannabis acids for measurement of selected biological activities-cytotoxicity against carcinogenic line B16 (musculus skin melanoma cells), cytotoxicity against Human Dermal Fibroblast (HDF) as a control non-carcinogenic line, antimicrobial activity against Candida albicans (DBM, 2186), Staphylococcus aureus (ATCC, 25923) and Salmonella enterica (CCM, 4420) and antioxidant activity using the ORAC (Oxygen Radical Absorbance Capacity) assay. 32 samples with a different profile of cannabinoids measured by HLPC were thus obtained and evaluated. The content of total flavonoids that could exert synergic action on bioactivity was measured by the spectrophotometric method. The hexane extracts showed higher antimicrobial activity, decarboxylation of the samples led to an increase in cytotoxicity against the human skin fibroblast line in most of the studied samples, and to decrease in the antioxidant effects of the decarboxylated extracts. The obtained findings suggest a therapeutic potential of the selected cannabis extracts in the treatment of skin cancer, skin wounds, or surgical wound healing.
Czech name
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Czech description
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Classification
Type
J<sub>ost</sub> - Miscellaneous article in a specialist periodical
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/FV30362" target="_blank" >FV30362: Nanofibre mesh with the admixture of cannabinoids for the treatment of wounds after extensive surgery</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
American Journal of Applied Sciences
ISSN
1554-3641
e-ISSN
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Volume of the periodical
19
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
68-77
UT code for WoS article
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EID of the result in the Scopus database
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