Oriented and reversible immobilisation of natural and recombinant photosystem II on gold microarray - potentials for use as biosensors for detection of herbicides

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F44555601%3A13430%2F04%3A00002624" target="_blank" >RIV/44555601:13430/04:00002624 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Oriented and reversible immobilisation of natural and recombinant photosystem II on gold microarray - potentials for use as biosensors for detection of herbicides
Original language description
Electrochemical on-chip deposition of proteins for further biodetection of several analytes by multiplexed chronoamperometry in a continuous flow m-chamber is presented. Recently, an original deposition procedure was developed obtaining oriented and reversible immobilisation of an engineered photosystem II with an histidine-tag and its on-chip purification from bacterial crude extracts. The electrochemically assisted method started with the deposition of cysteamin on gold or platinum surfaces and further chemical synthesis of a spacer with a Ni-NTA end capable to link the engineered proteins. This technique is applied on a m-chip assembled in a m-chamber with an histidine-tagged alkaline phosphatase. Electrochemical deposition allowed this protein to be addressed only on a specific electrode in the m-array in few minutes and under flow condition (flow-rate=10 ?l/min).
Czech name
Orientovaná a vratná imobilizace přirozeného a rekombinantního fotosystému II na povrchu micročipu
Czech description
Příspěvek popisuje současný stav vývoje elektrochemického biočipu pro detekci herbicidů.

Project
<a href="/en/project/GA522%2F03%2F0659" target="_blank" >GA522/03/0659: Improvement of photosynthetic biosenzors and bioassays for routine herbicide residues detection and non-target organisms risk assessment</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2004
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
The 9th national conference on sensors and microsystems AISEM
ISBN
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ISSN
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e-ISSN
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Number of pages
4
Pages from-to
88-91
Publisher name
Elsevier Science
Place of publication
Amsterdam
Event location
Ferrara, Italy
Event date
Feb 8, 2004
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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