All

What are you looking for?

All
Projects
Results
Organizations

Quick search

  • Projects supported by TA ČR
  • Excellent projects
  • Projects with the highest public support
  • Current projects

Smart search

  • That is how I find a specific +word
  • That is how I leave the -word out of the results
  • “That is how I can find the whole phrase”

The estimation of different ELISA procedures for serodiagnosis of human trichinellosis

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F44555601%3A13440%2F06%3A00003405" target="_blank" >RIV/44555601:13440/06:00003405 - isvavai.cz</a>

  • Alternative codes found

    RIV/62157124:16170/06:00000519

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    The estimation of different ELISA procedures for serodiagnosis of human trichinellosis

  • Original language description

    Introduction. The aim of presented study was to compare the usefulness of the results obtained by three ELISA procedures for Trichinella spp. diagnosis in human outbreaks. Materials and methods. All sera (cases and controls) were tested for anti-Trichinella antibodies (immunoglobulin G) using commercially available Novatec KIT and two other ELISA procedures based on excretory secretory (ES) antigens on Trichinella spiralis muscle larvae. The main differences in ELISA procedures were: the protein concentration in antigen, dilution of human serum samples, conjugate and the time of conjugate incubation. Additional differences were noticed in ES antigen preparation procedures as well as in T. spiralis isolates used in these procedures. Serum samples were obtained from 22 symptomatical patients from Poznań region (West Poland), geographic area where human outbreak had occurred. Control serum samples were obtained from 20 patients from an open population from a non endemic trichinellosis are

  • Czech name

    Zhodnocení různých ELISA metod pro sérodiagnostiku trichinelózy u lidí

  • Czech description

    Hlavním cílem předkládané studie bylo porovnání výsledků získaných 3 různými metodikami ELISA testu pro detekci trichinelózy u lidí. Krevní séra byla testována na anti-Trichinella protilátky použitím komerčního Novatec kitu a 2 různými ELISA metodami zapoužití E/S antigenu. Hlavní rozdíly v ELISA testech byly: koncentrace bílkovin v antigenu, zředění krevních sér, použitý konjugát a čas jeho inkubace. Krevní vzorky byly získány z 22 pacientů s příznaky trichinelózy z Poznaňské oblasti (Polsko). Výsledky odhalily u všech 3 metod různé hodnoty cut-off a tedy i pozitivity. Tyto odlišnosti mohou být významným faktorem z epidemiologického hlediska trichinelózy u lidí.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EG - Zoology

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GD524%2F03%2FH133" target="_blank" >GD524/03/H133: Biology, phylogeny and ecology of parasites at organismal and molecular level</a><br>

  • Continuities

    S - Specificky vyzkum na vysokych skolach

Others

  • Publication year

    2006

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Wiadomoąci Parazytologiczne

  • ISSN

    0043-5163

  • e-ISSN

  • Volume of the periodical

    52

  • Issue of the periodical within the volume

    3

  • Country of publishing house

    PL - POLAND

  • Number of pages

    8

  • Pages from-to

    231-238

  • UT code for WoS article

  • EID of the result in the Scopus database