Synthesis and characterization of biotin-silver-dendrimer nanocomposites for selective labeling in electrochemical immunoassays

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F44555601%3A13440%2F09%3A00004186" target="_blank" >RIV/44555601:13440/09:00004186 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Synthesis and characterization of biotin-silver-dendrimer nanocomposites for selective labeling in electrochemical immunoassays

Original language description

This report presents a synthesis of a novel electrochemical nanoparticle label with selective biorecognition properties based on a biotinylated silver-dendrimer nanocomposite (AgDNC). Two types of labels, a biotin-AgDNC (bio-AgDNC) and a biotinylated AgDNC with a polyethyleneglycol spacer (bio-PEG-AgDNC) were synthesized from a generation 7 (G7) hydroxyl-terminated ethylenediamine-core-type (2-carbon core) PAMAM dendrimer (DDM) by a N,N'-dicyclohexylcarbodiimide (DDC) biotin coupling and a NaBH4 silverreduction method. Synthesized conjugates were characterized by several analytical methods, as are UV-VIS, FTIR, AFM, TEM, ELISA, HABA assay and SPR. The results show that in both types of the biotinylated nanocomposites, the silver nanoparticles (AgNPs)are internalized and protected by a DMM polymer backbone (?type I NPs?) similarly to a nanocomposites synthesized from pure G7 PAMAM-OH dendrimer. The presence of a PEG spacer increases the internal volume of the polymer and enables highe

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EI - Biotechnology and bionics

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nanotechnology

ISSN

0957-4484

e-ISSN

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Volume of the periodical

20

Issue of the periodical within the volume

38

Country of publishing house

GB - UNITED KINGDOM

Number of pages

10

Pages from-to

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UT code for WoS article

000269356900001

EID of the result in the Scopus database

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