Phosphonium carbosilane dendrimers for biomedical applications - synthesis, characterization and cytotoxicity evaluation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F44555601%3A13440%2F17%3A43892841" target="_blank" >RIV/44555601:13440/17:43892841 - isvavai.cz</a>

Alternative codes found

RIV/67985858:_____/17:00473749

Result on the web

<a href="http://pubs.rsc.org/en/content/articlepdf/2017/ra/c7ra01845b" target="_blank" >http://pubs.rsc.org/en/content/articlepdf/2017/ra/c7ra01845b</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/c7ra01845b" target="_blank" >10.1039/c7ra01845b</a>

Result language

angličtina

Original language name

Phosphonium carbosilane dendrimers for biomedical applications - synthesis, characterization and cytotoxicity evaluation

Original language description

We report the synthesis and cytotoxicity evaluation of a completely new class of cationic carbosilane dendrimers functionalized with several different phosphonium peripheral groups and an ammonium functionalised one as a reference. The carbosilane dendrimers with NMe3, PMe3, P(Et-2)(2)(CH2)(3)OH, PBu3, P(C6H4-OMe)(3) and P(Ph)(3) peripheral substituents were synthesized, thoroughly characterized and modelled by computer simulations. The cytotoxicities of the dendrimers were investigated in vitro on three model cell lines (B14, BRL and NRK cells) by MTT and CV assay methods. Generally, the cytotoxicities of PMe3 carbosilane dendrimers were similar or slightly lower when compared with NMe3 dendrimers. The substitution of methyl groups in PMe3 carbosilane dendrimers with more hydrophobic and bulky alkyl substituents (PBu3 and P(Et-2)(2)(CH2)(3)OH dendrimers) resulted in an increase of cytotoxicity. The P(C6H4-OMe)(3) dendrimer showed exceptionally low cytotoxicity across all cell lines or assay methods used. Generally, phosphonium carbosilane dendrimers could represent a valuable alternative to ammonium ones in gene therapy applications due to comparable or lower cytotoxicities, the presence of positive charge for nucleic acid electrostatic binding and in the cases of P(C6H4-OMe)(3) and P(Ph)(3) dendrimers high potential of mitochondrial targeting.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

20501 - Materials engineering

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

RSC Advances

ISSN

2046-2069

e-ISSN

—

Volume of the periodical

2017

Issue of the periodical within the volume

7

Country of publishing house

GB - UNITED KINGDOM

Number of pages

21

Pages from-to

18724-18744

UT code for WoS article

000399005500067

EID of the result in the Scopus database

2-s2.0-85016408382