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Protocols for high-resolution respirometry experiments to test the activity of electron transfer system of pig brain mitochondria

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F44555601%3A13440%2F17%3A43893267" target="_blank" >RIV/44555601:13440/17:43893267 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216208:11110/17:10371630

  • Result on the web

    <a href="http://nopr.niscair.res.in/bitstream/123456789/43367/1/IJBB%2054%286%29%20258-272.pdf" target="_blank" >http://nopr.niscair.res.in/bitstream/123456789/43367/1/IJBB%2054%286%29%20258-272.pdf</a>

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Protocols for high-resolution respirometry experiments to test the activity of electron transfer system of pig brain mitochondria

  • Original language description

    The aim of our study was to develop protocols of high-resolution respirometry optimized for pig brain mitochondria. Optimal concentrations of components controlling oxidative phosphorylation were determined by titration of isolated mitochondria with digitonin, adenosine diphosphate, respiratory substrates (malate, pyruvate, succinate, TMPD), inhibitors (rotenone, malonate, antimycin, cyanide, azide, oligomycin), and uncoupler. Multiple substrate-uncoupler-inhibitor titration (SUIT) protocols were proposed for Complex I-, Complex II-, Complex I+II-, and Complex IV-linked respiration. Crude mitochondrial fraction and mitochondria purified on sucrose gradient were compared. We confirmed that pig brain mitochondria can serve as biological model for research of mitochondrial respiration.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10608 - Biochemistry and molecular biology

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2017

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Indian journal of biochemistry and biophysics

  • ISSN

    0301-1208

  • e-ISSN

  • Volume of the periodical

    2017

  • Issue of the periodical within the volume

    54

  • Country of publishing house

    IN - INDIA

  • Number of pages

    15

  • Pages from-to

    258-272

  • UT code for WoS article

    000424315600002

  • EID of the result in the Scopus database

    2-s2.0-85040256813