Protocols for high-resolution respirometry experiments to test the activity of electron transfer system of pig brain mitochondria
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F44555601%3A13440%2F17%3A43893267" target="_blank" >RIV/44555601:13440/17:43893267 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11110/17:10371630
Result on the web
<a href="http://nopr.niscair.res.in/bitstream/123456789/43367/1/IJBB%2054%286%29%20258-272.pdf" target="_blank" >http://nopr.niscair.res.in/bitstream/123456789/43367/1/IJBB%2054%286%29%20258-272.pdf</a>
DOI - Digital Object Identifier
—
Alternative languages
Result language
angličtina
Original language name
Protocols for high-resolution respirometry experiments to test the activity of electron transfer system of pig brain mitochondria
Original language description
The aim of our study was to develop protocols of high-resolution respirometry optimized for pig brain mitochondria. Optimal concentrations of components controlling oxidative phosphorylation were determined by titration of isolated mitochondria with digitonin, adenosine diphosphate, respiratory substrates (malate, pyruvate, succinate, TMPD), inhibitors (rotenone, malonate, antimycin, cyanide, azide, oligomycin), and uncoupler. Multiple substrate-uncoupler-inhibitor titration (SUIT) protocols were proposed for Complex I-, Complex II-, Complex I+II-, and Complex IV-linked respiration. Crude mitochondrial fraction and mitochondria purified on sucrose gradient were compared. We confirmed that pig brain mitochondria can serve as biological model for research of mitochondrial respiration.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Indian journal of biochemistry and biophysics
ISSN
0301-1208
e-ISSN
—
Volume of the periodical
2017
Issue of the periodical within the volume
54
Country of publishing house
IN - INDIA
Number of pages
15
Pages from-to
258-272
UT code for WoS article
000424315600002
EID of the result in the Scopus database
2-s2.0-85040256813