Study of non-covalent interactions on dendriplex formation: Influence of hydrophobic, electrostatic and hydrogen bonds interactions

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F44555601%3A13440%2F18%3A43893676" target="_blank" >RIV/44555601:13440/18:43893676 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0927776517308494?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0927776517308494?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.colsurfb.2017.12.009" target="_blank" >10.1016/j.colsurfb.2017.12.009</a>

Result language
angličtina
Original language name
Study of non-covalent interactions on dendriplex formation: Influence of hydrophobic, electrostatic and hydrogen bonds interactions
Original language description
tThe interaction of a double stranded small interference RNA (siRNA Nef) with cationic carbosilane den-drimers of generations 1-3 with two different ammonium functions at the periphery ([MINUS SIGN NMe2R]+, R = Me,(CH2)2OH) has been studied by experimental techniques (zeta potential, electrophoresis, single moleculepulling experiments) and molecular dynamic calculations. These studies state the presence of differentforces on dendriplex formation, depending on generation and type of ammonium group. Whilst for higherdendrimers electrostatic forces mainly drive the stability of dendriplexes, first generation compoundscan penetrate into siRNA strands due to the establishment of hydrophobic interactions. Finally, in the par-ticular case of first generation dendrimer [G1O3(NMe2(CH2)2OH))6]6+; the presence of hydroxyl groupsreinforces dendriplex stability by hydrogen bonds formation. However, since these small dendrimers donot cover the RNA, only higher generation derivatives protect RNA from degradation.
Czech name
—
Czech description
—

Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10610 - Biophysics

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)