A simple approach for fabrication of optical affinity-based bioanalytical microsystem on polymeric PEN foils

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F44555601%3A13440%2F18%3A43894071" target="_blank" >RIV/44555601:13440/18:43894071 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/18:00490109 RIV/60461373:22310/18:43917024
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0927776518300560?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0927776518300560?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.colsurfb.2018.01.048" target="_blank" >10.1016/j.colsurfb.2018.01.048</a>

Result language
angličtina
Original language name
A simple approach for fabrication of optical affinity-based bioanalytical microsystem on polymeric PEN foils
Original language description
tHerein, we report a novel concept of low-cost flexible platform for fluorescence-based biosensor. Thesurface of polyethylene naphthalate (PEN) foil was exposed to KrF excimer laser through a photolito-graphic contact mask. Laser initiated surface modification resulted in micro-patterned areas with surfacefunctional groups available for localized covalent immobilization of biotin. High affinity binding protein(albumin-binding domain (ABD) of protein G, Streptococcus G148) recognizing human serum albumin(HSA), genetically fused with streptavidin (SA-ABDwt), was immobilized on the micro-patterned surfacethrough biotin-streptavidin coupling. Fluorescently labelled HSA analyte was detected in several block-ing environments, in 1% bovine serum albumin (BSA) and 6% fetal serum albumin (FBS), respectively. Weconclude that the presented novel concept enabled us to micropattern functional biosensing layers onthe surface of PEN foil in a fast and easy way. It brings all necessary aspects for continuous roll-to-rollfabrication of low-cost optical bioanalytical devices.
Czech name
—
Czech description
—

Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
20501 - Materials engineering

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)