Dramatic loss of microbial viability in bentonite exposed to heat and gamma radiation: implications for deep geological repository
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46356088%3A_____%2F24%3AN0000016" target="_blank" >RIV/46356088:_____/24:N0000016 - isvavai.cz</a>
Alternative codes found
RIV/46747885:24620/24:00012911
Result on the web
<a href="https://link.springer.com/article/10.1007/s11274-024-04069-w" target="_blank" >https://link.springer.com/article/10.1007/s11274-024-04069-w</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11274-024-04069-w" target="_blank" >10.1007/s11274-024-04069-w</a>
Alternative languages
Result language
angličtina
Original language name
Dramatic loss of microbial viability in bentonite exposed to heat and gamma radiation: implications for deep geological repository
Original language description
Bentonite is an integral part of the engineered barrier system (EBS) in deep geological repositories (DGR) for nuclear waste, but its indigenous microorganisms may jeopardize long-term EBS integrity. To predict microbial activity in DGRs, it is essential to understand microbial reactions to the early hot phase of DGR evolution. Two bentonites (BCV and MX-80) with varied bentonite/water ratios and saturation levels (compacted to 1600 kg.m− 3 dry density/powder/suspension), were subjected to heat (90–150 °C) and irradiation (0.4 Gy.h− 1) in the long-term experiments (up to 18 months). Molecular-genetic, microscopic, and cultivation-based techniques assessed microbial survivability. Exposure to 90 °C and 150 °C notably diminished microbial viability, irrespective of bentonite form, with negligible impacts from irradiation or sample type compared to temperature. Bentonite powder samples exhibited microbial recovery after 90 °C heating for up to 6 months but not 12 months in most cases; exposure to 150 °C had an even stronger effect. Further long-term experiments at additional temperatures combined with the mathematical prediction of temperature evolution in DGR are recommended to validate the possible evolution and spatial distribution of microbially depleted zones in bentonite buffer around the waste canisters and refine predictions of microbial effects over time in the DGR.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
20801 - Environmental biotechnology
Result continuities
Project
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Continuities
R - Projekt Ramcoveho programu EK
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
World journal of microbiology and biotechnology
ISSN
0959-3993
e-ISSN
1573-0972
Volume of the periodical
40
Issue of the periodical within the volume
9
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
19
Pages from-to
1-19
UT code for WoS article
001266641500001
EID of the result in the Scopus database
2-s2.0-85198120848