Cytokine response to the RSV antigen delivered by dendritic cell-directed vaccination in congenic chicken lines
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46356606%3A_____%2F17%3AN0000002" target="_blank" >RIV/46356606:_____/17:N0000002 - isvavai.cz</a>
Result on the web
<a href="https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-017-0423-8" target="_blank" >https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-017-0423-8</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s13567-017-0423-8" target="_blank" >10.1186/s13567-017-0423-8</a>
Alternative languages
Result language
angličtina
Original language name
Cytokine response to the RSV antigen delivered by dendritic cell-directed vaccination in congenic chicken lines
Original language description
Systems of antigen delivery into antigen-presenting cells represent an important novel strategy in chicken vaccine development. In this study, we verified the ability of Rous sarcoma virus (RSV) antigens fused with streptavidin to be targeted by specific biotinylated monoclonal antibody (anti-CD205) into dendritic cells and induce virus-specific protective immunity. The method was tested in four congenic lines of chickens that are either resistant or susceptible to the progressive growth of RSV-induced tumors. Our analyses confirmed that the biot-anti-CD205-SA-FITC complex was internalized by chicken splenocytes. In the cytokine expression profile, several significant differences were evident between RSV-challenged progressor and regressor chicken lines. A significant up-regulation of IL-2, IL-12, IL-15, and IL-18 expression was detected in immunized chickens of both regressor and progressor groups. Of these cytokines, IL-2 and IL-12 were most up-regulated 14 days post-challenge (dpc), while IL-15 and IL-18 were most up-regulated at 28 dpc. On the contrary, IL-10 expression was significantly down-regulated in all immunized groups of progressor chickens at 14 dpc. We detected significant up-regulation of IL-17 in the group of immunized progressors. LITAF down-regulation with iNOS up-regulation was especially observed in the progressor group of immunized chickens that developed large tumors. Based on the increased expression of cytokines specific for activated dendritic cells, we conclude that our system is able to induce partial stimulation of specific cell types involved in cell-mediated immunity.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40301 - Veterinary science
Result continuities
Project
<a href="/en/project/QJ1210041" target="_blank" >QJ1210041: New type of vaccine against chicken viral diseases</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Veterinary Research
ISSN
1297-9716
e-ISSN
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Volume of the periodical
48
Issue of the periodical within the volume
18
Country of publishing house
FR - FRANCE
Number of pages
14
Pages from-to
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UT code for WoS article
000399675200001
EID of the result in the Scopus database
2-s2.0-85016958853