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Cytokine response to the RSV antigen delivered by dendritic cell-directed vaccination in congenic chicken lines

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46356606%3A_____%2F17%3AN0000002" target="_blank" >RIV/46356606:_____/17:N0000002 - isvavai.cz</a>

  • Result on the web

    <a href="https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-017-0423-8" target="_blank" >https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-017-0423-8</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1186/s13567-017-0423-8" target="_blank" >10.1186/s13567-017-0423-8</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Cytokine response to the RSV antigen delivered by dendritic cell-directed vaccination in congenic chicken lines

  • Original language description

    Systems of antigen delivery into antigen-presenting cells represent an important novel strategy in chicken vaccine development. In this study, we verified the ability of Rous sarcoma virus (RSV) antigens fused with streptavidin to be targeted by specific biotinylated monoclonal antibody (anti-CD205) into dendritic cells and induce virus-specific protective immunity. The method was tested in four congenic lines of chickens that are either resistant or susceptible to the progressive growth of RSV-induced tumors. Our analyses confirmed that the biot-anti-CD205-SA-FITC complex was internalized by chicken splenocytes. In the cytokine expression profile, several significant differences were evident between RSV-challenged progressor and regressor chicken lines. A significant up-regulation of IL-2, IL-12, IL-15, and IL-18 expression was detected in immunized chickens of both regressor and progressor groups. Of these cytokines, IL-2 and IL-12 were most up-regulated 14 days post-challenge (dpc), while IL-15 and IL-18 were most up-regulated at 28 dpc. On the contrary, IL-10 expression was significantly down-regulated in all immunized groups of progressor chickens at 14 dpc. We detected significant up-regulation of IL-17 in the group of immunized progressors. LITAF down-regulation with iNOS up-regulation was especially observed in the progressor group of immunized chickens that developed large tumors. Based on the increased expression of cytokines specific for activated dendritic cells, we conclude that our system is able to induce partial stimulation of specific cell types involved in cell-mediated immunity.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    40301 - Veterinary science

Result continuities

  • Project

    <a href="/en/project/QJ1210041" target="_blank" >QJ1210041: New type of vaccine against chicken viral diseases</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2017

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Veterinary Research

  • ISSN

    1297-9716

  • e-ISSN

  • Volume of the periodical

    48

  • Issue of the periodical within the volume

    18

  • Country of publishing house

    FR - FRANCE

  • Number of pages

    14

  • Pages from-to

  • UT code for WoS article

    000399675200001

  • EID of the result in the Scopus database

    2-s2.0-85016958853