Male fertility restored by transplanting primordial germ cells into testes: A new way towards efficient transgenesis in chicken
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46356606%3A_____%2F17%3AN0000004" target="_blank" >RIV/46356606:_____/17:N0000004 - isvavai.cz</a>
Alternative codes found
RIV/68378050:_____/17:00487318
Result on the web
<a href="https://www.nature.com/articles/s41598-017-14475-w" target="_blank" >https://www.nature.com/articles/s41598-017-14475-w</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-017-14475-w" target="_blank" >10.1038/s41598-017-14475-w</a>
Alternative languages
Result language
angličtina
Original language name
Male fertility restored by transplanting primordial germ cells into testes: A new way towards efficient transgenesis in chicken
Original language description
The ongoing progress in primordial germ cell derivation and cultivation is opening new ways in reproductive biotechnology. This study tested whether functional sperm cells can be matured from genetically manipulated primordial germ cells after transplantation in adult testes and used to restore fertility. We show that spermatogenesis can be restored after mCherry-expressing or GFP-expressing primordial germ cells are transplantated into the testes of sterilized G(0) roosters and that mCherry-positive or GFP-positive non-chimeric transgenic G(1) offspring can be efficiently produced. Compared with the existing approaches to primordial germ cell replacement, this new technique eliminates the germ line chimerism of G(0) roosters and is, therefore, faster, more efficient and requires fewer animals. Furthermore, this is the only animal model, where the fate of primordial germ cells in infertile recipients can be studied.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10604 - Reproductive biology (medical aspects to be 3)
Result continuities
Project
<a href="/en/project/GA15-23993S" target="_blank" >GA15-23993S: Gene targeting in chicken and the resistance to newly emerging retroviruses</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Scientific Reports
ISSN
2045-2322
e-ISSN
2045-2322
Volume of the periodical
7
Issue of the periodical within the volume
14246
Country of publishing house
GB - UNITED KINGDOM
Number of pages
9
Pages from-to
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UT code for WoS article
000413907000069
EID of the result in the Scopus database
2-s2.0-85032461218