DRAWING FIBROUS SCAFFOLDS WITH PRECISE STRUCTURE FOR TISSUE ENGINEERING

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24210%2F14%3A00003522" target="_blank" >RIV/46747885:24210/14:00003522 - isvavai.cz</a>

Alternative codes found

RIV/46747885:24410/14:00003522

Result on the web

<a href="http://www.nanocon.eu/files/uploads/01/NANOCON" target="_blank" >http://www.nanocon.eu/files/uploads/01/NANOCON</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

DRAWING FIBROUS SCAFFOLDS WITH PRECISE STRUCTURE FOR TISSUE ENGINEERING

Original language description

This work is focused on evaluation of scaffolds made by drawing technique for the cell adhesion, viability and proliferation. With drawing we are able to produce very precise fibres with specified orientation from different polymers (PCL, PVA, PVB and others). This characteristic could be an advantage in tissues with ordered pattern. In the primary study the handmade fibres were tested for the cell growth and the results showed the potential of the cells to overgrow the gaps between the fibres. On account of these results further tests were necessary to be done with the precise machine-made fibrous scaffolds. The machine-made fibres are thinner, the gaps between the fibres are smaller and the whole structure is denser. In this study we focused on a planar scaffolds with fibres ordered either in one, in two or in three directions. Mice fibroblasts (3T3 Swiss Albino) were used for the in vitro experiment. The scaffolds were tested at day 1, 3, 7, 14, 21 and 28 after cell seeding. MTT assay, fluorescent microscopy and SEM analysis were used for the evaluation of cell adhesion, viability and proliferation. The results from microscopy analysis as well as from the MTT assay show, that the machine-made scaffolds are capable of supporting cellular attachment and proliferation during our in vitro tests. The scaffolds with crossed fibres show better results compared to fibres ordered in one direction. Nevertheless the one-direction fibres have a great potential in many tissue engineering applications, e.g. for muscles or neural tissue..

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

FP - Other medical fields

OECD FORD branch

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Project

<a href="/en/project/ED3.1.00%2F14.0306" target="_blank" >ED3.1.00/14.0306: Innovative products and environmental technologies</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

NANOCON 2014 - Conference Proceedings, 6th International Conference

ISBN

978-80-87294-53-6

ISSN

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e-ISSN

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Number of pages

6

Pages from-to

508-513

Publisher name

TANGER LTD, OSTRAVA

Place of publication

Ostrava, CZECH REPUBLIC

Event location

Brno, CZECH REPUBLIC

Event date

Jan 1, 2014

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

000350636300087