Nanosecond compressive fluorescence lifetime microscopy imaging via the RATS method with a direct reconstruction of lifetime maps
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24220%2F23%3A00010758" target="_blank" >RIV/46747885:24220/23:00010758 - isvavai.cz</a>
Alternative codes found
RIV/61389021:_____/23:00583129
Result on the web
<a href="https://opg.optica.org/oe/fulltext.cfm?uri=oe-31-4-5181&id=525632&ibsearch=false" target="_blank" >https://opg.optica.org/oe/fulltext.cfm?uri=oe-31-4-5181&id=525632&ibsearch=false</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1364/OE.474453" target="_blank" >10.1364/OE.474453</a>
Alternative languages
Result language
angličtina
Original language name
Nanosecond compressive fluorescence lifetime microscopy imaging via the RATS method with a direct reconstruction of lifetime maps
Original language description
The RAndom Temporal Signals (RATS) method has proven to be a useful and versatile method for measuring photoluminescence (PL) dynamics and fluorescence lifetime imaging (FLIM). Here, we present two fundamental development steps in the method. First, we demonstrate that by using random digital laser modulation in RATS, it is possible to implement the measurement of PL dynamics with temporal resolution in units of nanoseconds. Secondly, we propose an alternative approach to evaluating FLIM measurements based on a single-pixel camera experiment. In contrast to the standard evaluation, which requires a lengthy iterative reconstruction of PL maps for each timepoint, here we use a limited set of predetermined PL lifetimes and calculate the amplitude maps corresponding to each lifetime. The alternative approach significantly saves post-processing time and, in addition, in a system with noise present, it shows better stability in terms of the accuracy of the FLIM spectrogram. Besides simulations that confirmed the functionality of the extension, we implemented the new advancements into a microscope optical setup for mapping PL dynamics on the micrometer scale. The presented principles were also verified experimentally by mapping a LuAG:Ce crystal surface.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10306 - Optics (including laser optics and quantum optics)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Optics Express
ISSN
1094-4087
e-ISSN
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Volume of the periodical
31
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
19
Pages from-to
5181-5199
UT code for WoS article
000942082400004
EID of the result in the Scopus database
2-s2.0-85147323918