Histological mapping of porcine carotid arteries — An animal model for the assessment of artificial conduits suitable for coronary bypass grafting in humans

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24410%2F20%3A00006802" target="_blank" >RIV/46747885:24410/20:00006802 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/20:10401791 RIV/00216208:11130/20:10401791 RIV/00216208:11140/20:10401791 RIV/00064211:_____/20:W0000030 RIV/00023001:_____/20:00078738

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0940960219301384" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0940960219301384</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aanat.2019.151434" target="_blank" >10.1016/j.aanat.2019.151434</a>

Result language

angličtina

Original language name

Histological mapping of porcine carotid arteries — An animal model for the assessment of artificial conduits suitable for coronary bypass grafting in humans

Original language description

© 2019 Elsevier B.V. Background: Using animal models in experimental medicine requires mapping of their anatomical variability. Porcine common carotid arteries (CCA) are often preferred for the preclinical testing of vascular grafts due to their anatomical and physiological similarity to human small-diameter arteries. Comparing the microscopic structure of animal model organs to their human counterparts reveals the benefits and limitations of translational medicine. Methods: Using quantitative histology and stereology, we performed an extensive mapping of the regional proximodistal differences in the fractions of elastin, collagen, and smooth muscle actin as well as the intima-media and wall thicknesses among 404 segments (every 1 cm) of porcine CCAs collected from male and female pigs (n = 21). We also compared the microscopic structure of porcine CCAs with segments of human coronary arteries and one of the preferred arterial conduits used for the coronary artery bypass grafting (CABG), namely, the internal thoracic artery (ITA) (n = 21 human cadavers). Results: The results showed that the histological structure of left and right porcine CCA can be considered equivalent, provided that gross anatomical variations of the regular branching patterns are excluded. The proximal elastic carotid (51.2% elastin, 4.2% collagen, and 37.2% actin) transitioned to more muscular middle segments (23.5% elastin, 4.9% collagen, 54.3% actin) at the range of 2–3 centimeters and then to even more muscular distal segments (17.2% elastin, 4.9% collagen, 64.0% actin). The resulting morphometric data set shows the biological variability of the artery and is made available for biomechanical modeling and for performing a power analysis and calculating the minimum number of samples per group when planning further experiments with this widely used large animal model. Conclusions: Comparison of porcine carotids with human coronary arteries and ITA revealed the benefits and the limitations of using porcine CCAs as a valid model for testing bioengineered small-diameter CABG vascular conduits. Morphometry of human coronary arteries and ITA provided more realistic data for tailoring multilayered artificial vascular prostheses and the ranges of values within which the conduits should be tested in the future. Despite their limitations, porcine CCAs remain a widely used and well-characterized large animal model that is available for a variety of experiments in vascular surgery.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30201 - Cardiac and Cardiovascular systems

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Annals of Anatomy - Anatomischer Anzeiger

ISSN

0940-9602

e-ISSN

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Volume of the periodical

228

Issue of the periodical within the volume

March

Country of publishing house

DE - GERMANY

Number of pages

20

Pages from-to

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UT code for WoS article

000539110400005

EID of the result in the Scopus database

2-s2.0-85075145206