Application of laser-induced breakdown cavitation bubbles for cell lysis in vitro

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24620%2F19%3A00006990" target="_blank" >RIV/46747885:24620/19:00006990 - isvavai.cz</a>

Result on the web

<a href="https://innovareacademics.in/journals/index.php/ijap/article/view/35860" target="_blank" >https://innovareacademics.in/journals/index.php/ijap/article/view/35860</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.22159/ijap.2019.v11s5.T3059" target="_blank" >10.22159/ijap.2019.v11s5.T3059</a>

Result language

angličtina

Original language name

Application of laser-induced breakdown cavitation bubbles for cell lysis in vitro

Original language description

Objective: Understanding the basic mechanism of the cavitation bubble action on living cells as a crucial step of development and application of sophisticated methods based on controlled cavitation in cell behaviour manipulation. Optimisation of parameters in order to expand cell lysis region created by a single bubble. Methods: The cavitation bubbles are generated by the laser-induced breakdown method. The impact of controlled cavitation bubble on the biological system is synchronously monitored under a microscope and recorded. Visualization of the cavitation bubble course is monitored by a high-speed camera. The impact of technology on the healthy confluent cell layer is verified. Evaluation of the cavitation bubbles` effect on cells in real time and by subsequent analysis of the cell lysis region and impact of the cavitation bubble on cell viability is carried out by optical visualization and life/ dead fluorescence staining. Results: Cavitation bubble induced in distance of 1.5 mm from the cell surface overcomes properties of sessile bubble and enables to create cell lysis region over 1000 µm in diameter due to transient shear stress produced by liquid displaced by the bubble expansion. Conclusion: Cell lysis region is strongly dependent on the spot laser energy (SLE) and the bubble induction distance from cells. This knowledge is crucial for application in chemical free cell lysis in vitro, wound induction for experimental purposes and cell layers patterning in desired scale.

Czech name

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Czech description

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Type

J_SC - Article in a specialist periodical, which is included in the SCOPUS database

CEP classification

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OECD FORD branch

10610 - Biophysics

Project

<a href="/en/project/EF16_019%2F0000843" target="_blank" >EF16_019/0000843: Hybrid Materials for Hierarchical Structure</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Applied Pharmaceutics

ISSN

09757058

e-ISSN

—

Volume of the periodical

11

Issue of the periodical within the volume

5

Country of publishing house

IN - INDIA

Number of pages

5

Pages from-to

186-190

UT code for WoS article

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EID of the result in the Scopus database

2-s2.0-85073527866