Chapter 4 - Molecular techniques for understanding microbial abundance and activity in clay barriers used for geodisposal
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24620%2F20%3A00008706" target="_blank" >RIV/46747885:24620/20:00008706 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1016/B978-0-12-818695-4.00004-6" target="_blank" >https://doi.org/10.1016/B978-0-12-818695-4.00004-6</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/B978-0-12-818695-4.00004-6" target="_blank" >10.1016/B978-0-12-818695-4.00004-6</a>
Alternative languages
Result language
angličtina
Original language name
Chapter 4 - Molecular techniques for understanding microbial abundance and activity in clay barriers used for geodisposal
Original language description
Clays are commonly used in design concepts for geological disposal of nuclear waste. It is thus essential to identify and quantify microbial communities in clay-rich samples to study microbial processes during geological disposal. Although advances in culture-independent techniques have enabled detailed studies of microbial communities in diverse ecosystems, the efficiency and sensitivity of these molecular techniques depend on characteristics of the environment studied. Moreover, the outcome of nucleic acid-based approaches depends on the extraction method, primer specificity, PCR amplification, sequencing artifacts, and downstream bioinformatic analyses. Clays are recalcitrant to DNA extraction and are challenging for analysis by standard techniques using viability stains and measurement of metabolic activity. This chapter explores the impact of various sequencing and bioinformatic pipelines used for 16S rRNA gene profiling of microbial communities and compares the efficiency of different DNA extraction methods from clay. Moreover, we also discuss non-DNA-based techniques used to assess microbial activity and viability in clay samples.
Czech name
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Czech description
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Classification
Type
C - Chapter in a specialist book
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
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Continuities
R - Projekt Ramcoveho programu EK
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Book/collection name
The Microbiology of Nuclear Waste Disposal
ISBN
978-0-12-818695-4
Number of pages of the result
26
Pages from-to
71-96
Number of pages of the book
355
Publisher name
Elsevier
Place of publication
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UT code for WoS chapter
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