Transcriptomic analysis and cellular responses to nanoscale zero-valent iron in green microalga Raphidocelis subcapitata

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24620%2F24%3A00012908" target="_blank" >RIV/46747885:24620/24:00012908 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1016/j.ecoenv.2024.117194" target="_blank" >https://doi.org/10.1016/j.ecoenv.2024.117194</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ecoenv.2024.117194" target="_blank" >10.1016/j.ecoenv.2024.117194</a>

Result language

angličtina

Original language name

Transcriptomic analysis and cellular responses to nanoscale zero-valent iron in green microalga Raphidocelis subcapitata

Original language description

Nanoscale zero valent iron (nZVI) is used to remediate aquifers polluted by organochlorines or heavy metals and was also suggested to eliminate harmful algal blooms. nZVI can therefore affect microorganisms in the vicinity of the application area, including microalgae. However, studies on early transcriptomic effects of microalgae after exposure to nZVI are rare. Here, we described the early physiological and transcriptomic response of the freshwater ecological indicator green microalga, Raphidocelis subcapitata ATCC 22662, to 100 mg/L of reactive nZVI and non-reactive nano-magnetite (nFe3O4). The combined effect of shading and the release of total iron from nZVI posed a short-term inhibition effect leading to 15 % of deformed cells and cytosol leakage, while cells viability increased after 24 h. nZVI triggered a more pronounced transcriptomic response with (7380 differentially expressed genes [DEGs]) compared to nFe3O4 (4601 DEGs) after 1 h. nZVI, but not nFe3O4 increased the expression of genes function in DNA repair and replication, while deactivated carbohydrate-energy metabolisms, mitochondria signaling, and transmembrane ion transport. This study highlights an early fate assessment of algal cells under nZVI and nFe3O4 exposure using next-generation risk assessment methods and will serve as valuable information for safe and sustainable application of nZVI in water remediation.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10511 - Environmental sciences (social aspects to be 5.7)

Project

<a href="/en/project/EF16_019%2F0000843" target="_blank" >EF16_019/0000843: Hybrid Materials for Hierarchical Structure</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY

ISSN

0147-6513

e-ISSN

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Volume of the periodical

286

Issue of the periodical within the volume

NOV 1

Country of publishing house

US - UNITED STATES

Number of pages

14

Pages from-to

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UT code for WoS article

001344151600001

EID of the result in the Scopus database

2-s2.0-85206982449