Analysis of nanomaterial biocoronas in biological and environmental surroundings
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24620%2F24%3A00013037" target="_blank" >RIV/46747885:24620/24:00013037 - isvavai.cz</a>
Result on the web
<a href="https://www.researchgate.net/publication/382507202_Analysis_of_nanomaterial_biocoronas_in_biological_and_environmental_surroundings" target="_blank" >https://www.researchgate.net/publication/382507202_Analysis_of_nanomaterial_biocoronas_in_biological_and_environmental_surroundings</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41596-024-01009-8" target="_blank" >10.1038/s41596-024-01009-8</a>
Alternative languages
Result language
angličtina
Original language name
Analysis of nanomaterial biocoronas in biological and environmental surroundings
Original language description
A biomolecular coating, or biocorona, forms on the surface of engineered nanomaterials (ENMs) immediately as they enter biological or environmental systems, defining their biological and environmental identity and influencing their fate and performance. This biomolecular layer includes proteins (the protein corona) and other biomolecules, such as nucleic acids and metabolites. To ensure a meaningful and reproducible analysis of the ENMs-associated biocorona, it is essential to streamline procedures for its preparation, separation, identification and characterization, so that studies in different labs can be easily compared, and the information collected can be used to predict the composition, dynamics and properties of biocoronas acquired by other ENMs. Most studies focus on the protein corona as proteins are easier to monitor and characterize than other biomolecules and play crucial roles in receptor engagement and signaling; however, metabolites play equally critical roles in signaling. Here we describe how to reproducibly prepare and characterize biomolecule-coated ENMs, noting especially the steps that need optimization for different types of ENMs. The structure and composition of the biocoronas are characterized using general methods (transmission electron microscopy, dynamic light scattering, capillary electrophoresis–mass spectrometry and liquid chromatography–mass spectrometry) as well as advanced techniques, such as transmission electron cryomicroscopy, synchrotron-based X-ray absorption near edge structure and circular dichroism. We also discuss how to use molecular dynamic simulation to study and predict the interaction between ENMs and biomolecules and the resulting biocorona composition. The application of this protocol can provide mechanistic insights into the formation, composition and evolution of the ENM biocorona, ultimately facilitating the biomedical and agricultural application of ENMs and a better understanding of their impact in the environment.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nature Protocols
ISSN
1754-2189
e-ISSN
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Volume of the periodical
19
Issue of the periodical within the volume
10
Country of publishing house
DE - GERMANY
Number of pages
48
Pages from-to
3000-3047
UT code for WoS article
001275227900001
EID of the result in the Scopus database
2-s2.0-85199283279