ŠVÁBOVÁ, L., GRIGA, M., HORÁČEK, J., ONDŘEJ, V., ŠERHANTOVÁ, V.: Methodological aspect of pea (Pisum sativum L.) genetic transformation.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F48392952%3A_____%2F03%3A00007139" target="_blank" >RIV/48392952:_____/03:00007139 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—
Alternative languages
Result language
angličtina
Original language name
ŠVÁBOVÁ, L., GRIGA, M., HORÁČEK, J., ONDŘEJ, V., ŠERHANTOVÁ, V.: Methodological aspect of pea (Pisum sativum L.) genetic transformation.
Original language description
Three basic regeneration protocols were used in a set of experiments focused on the development of reliable, routine and genotype-independent transformation method in pea:(1) multiple shoot culture (MSC) derived from axillary meristems, (2) somatic embryogenesis (SE) from apical meristems,(3) trimmed germinating seeds (TGS). The indirect transformation via Agrobacterium tumefaciens (EHA 105) carrying plasmids pBIN19 and pGT89 was used for the basic studies. SE-based Agrobacterium transformation approachresulted in the lowest transformation efficiency (TE)1-2%, TE of MSC and TGS was 18 % and 30 % in T0 generation, respectively. SE regeneration protocol was also used for the experiments with direct gene transfer via particle bombardement with plasmid pDB1. Variants of pressure / grid combinations were studied for optimal particle gun setting. The efficiency of transformation via particle bombardement was 0,5-1,4 %.
Czech name
—
Czech description
—
Classification
Type
D - Article in proceedings
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
—
Result continuities
Project
<a href="/en/project/ME%20433" target="_blank" >ME 433: Genetic engineering and molecular biology in grain legumes</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2003
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Plant Biotechnology: Progress and Developments, Book of Abstracts
ISBN
80-89088-15-5
ISSN
—
e-ISSN
—
Number of pages
1
Pages from-to
89
Publisher name
Institute of Plant Genetics and Biotechnology SAS
Place of publication
Nitra, Slovakia
Event location
Stará Lesná, Slovakia
Event date
Sep 7, 2003
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
—