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Cost-effective straightforward method for captured whole mitogenome sequencing of ancient DNA

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F48511005%3A_____%2F20%3AN0000024" target="_blank" >RIV/48511005:_____/20:N0000024 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216224:14310/21:00120836

  • Result on the web

    <a href="https://doi.org/10.1016/j.forsciint.2020.110638" target="_blank" >https://doi.org/10.1016/j.forsciint.2020.110638</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.forsciint.2020.110638" target="_blank" >10.1016/j.forsciint.2020.110638</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Cost-effective straightforward method for captured whole mitogenome sequencing of ancient DNA

  • Original language description

    Working with mitochondrial DNA from highly degraded samples is challenging. We present a whole mitogenome Illumina-based sequencing method suitable for highly degraded samples. The method makes use of double-stranded library preparation with hybridization-based target enrichment. The aim of the study was to implement a new user-friendly method for analysing many ancient DNA samples at a low cost. The method combines the Swift 2S™ Turbo library preparation kit and xGen® panel for mitogenome enrichment. Swift allows to use low input of aDNA and own adapters and primers, handles inhibitors well, and has only two purification steps. xGen is straightforward to use and is able to leverage already pooled libraries. Given the ancient DNA is more challenging to work with, the protocol was developed with several improvements, especially multiplying DNA input in case of low concentration DNA extractions followed by AMPure® beads size selection and real-time pre-capture PCR monitoring in order to avoid cycle-optimization step. Nine out of eleven analysed samples successfully retrieved mitogenomes. Hence, our method provides an effective analysis of whole mtDNA, and has proven to be fast, cost-effective, straightforward, with utilisation in population-wide research of burial sites.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>SC</sub> - Article in a specialist periodical, which is included in the SCOPUS database

  • CEP classification

  • OECD FORD branch

    10608 - Biochemistry and molecular biology

Result continuities

  • Project

    <a href="/en/project/LM2015091" target="_blank" >LM2015091: National Center for Medical Genomic</a><br>

  • Continuities

    V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Others

  • Publication year

    2020

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Forensic Science International

  • ISSN

    0379-0738

  • e-ISSN

  • Volume of the periodical

    319

  • Issue of the periodical within the volume

    110638

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    14

  • Pages from-to

  • UT code for WoS article

  • EID of the result in the Scopus database

    2-s2.0-85097778404