Cost-effective straightforward method for captured whole mitogenome sequencing of ancient DNA

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F48511005%3A_____%2F20%3AN0000024" target="_blank" >RIV/48511005:_____/20:N0000024 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14310/21:00120836

Result on the web

<a href="https://doi.org/10.1016/j.forsciint.2020.110638" target="_blank" >https://doi.org/10.1016/j.forsciint.2020.110638</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.forsciint.2020.110638" target="_blank" >10.1016/j.forsciint.2020.110638</a>

Result language

angličtina

Original language name

Cost-effective straightforward method for captured whole mitogenome sequencing of ancient DNA

Original language description

Working with mitochondrial DNA from highly degraded samples is challenging. We present a whole mitogenome Illumina-based sequencing method suitable for highly degraded samples. The method makes use of double-stranded library preparation with hybridization-based target enrichment. The aim of the study was to implement a new user-friendly method for analysing many ancient DNA samples at a low cost. The method combines the Swift 2S™ Turbo library preparation kit and xGen® panel for mitogenome enrichment. Swift allows to use low input of aDNA and own adapters and primers, handles inhibitors well, and has only two purification steps. xGen is straightforward to use and is able to leverage already pooled libraries. Given the ancient DNA is more challenging to work with, the protocol was developed with several improvements, especially multiplying DNA input in case of low concentration DNA extractions followed by AMPure® beads size selection and real-time pre-capture PCR monitoring in order to avoid cycle-optimization step. Nine out of eleven analysed samples successfully retrieved mitogenomes. Hence, our method provides an effective analysis of whole mtDNA, and has proven to be fast, cost-effective, straightforward, with utilisation in population-wide research of burial sites.

Czech name

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Czech description

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Type

J_SC - Article in a specialist periodical, which is included in the SCOPUS database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/LM2015091" target="_blank" >LM2015091: National Center for Medical Genomic</a><br>

Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Forensic Science International

ISSN

0379-0738

e-ISSN

—

Volume of the periodical

319

Issue of the periodical within the volume

110638

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

14

Pages from-to

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UT code for WoS article

—

EID of the result in the Scopus database

2-s2.0-85097778404