Impact of BCS of dairy cows on quality of embryos

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F49608851%3A_____%2F13%3A%230000594" target="_blank" >RIV/49608851:_____/13:#0000594 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Impact of BCS of dairy cows on quality of embryos
Original language description
The aim of the study was to examine quality of in vivo-derived cattle embryos. The embryos were recovered from superovulated Holstein cows (n = 59), classified for body condition score (BCS) according to a 5-point scale (BCS2- n = 17; BCS3- n = 33; BCS4-n = 9), on 6th day after the first insemination by a non-surgical flushing of the uterine horns. The embryos were morphologically evaluated for developmental stage (morula, blastocyst, fragmented/degenerated) and analyzed for apoptosis (TUNEL), total cell number (DAPI), actin cytoskeleton quality (phalloidine-TRITC) and cell ultrastructure (transmission electron microscopy). The highest embryo yield (69.3%) was obtained in the BCS3 group, but with the BCS elevation the embryo yield decreased and the last embryo yield (35%) was obtained in the BCS4. The lower embryo cell count and higher number of embryos with cytoplasmic fragmentation were recorded in the BCS4, compared to BCS2 or BCS3 groups. Apoptotic index was highest in the BCS4 em
Czech name
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Czech description
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Project
<a href="/en/project/QI91A061" target="_blank" >QI91A061: Influence of different metabolic activity of organisms on biological and fertilizing characteristics of gametes in dairy cattle.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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